Table 3 Plasmids used in this study

pACLacI

pACYC184 derivative with lacI^q; Cm^R

This study

pASK75

vector, P/O _tet , tetR, ColEI ori; Ap^R

[60]

pASKSurA^a

surA gene in pASK75; Ap^R

[2]

pASKSurAN-Ct^b

surAN-Ct fusion from pSurAN-Ct [2] in pASK75; Ap^R

This study

pASKssPpiD

surA signal sequence-his₆-ppiD (codons 34-623) fusion in pASK75; Ap^R

This study

pASKssPpiDΔParv

pASKssPpiDΔ252-355; Ap^R

This study

pΩSurA

Ω::spec-P _Llac-O1 surA in pUC18; Ap^R; Spec^R

This study

pMP1

skp gene region of E. coli MC1061 (corresponding to nucleotides 199495-201937 of the E. coli MG1655 genome^c) in pSU18; Cm^R

Gross laboratory

pPLT13

mini-F carrying lacI^q; Kan^R

[61]

pPpiD

ppiD gene and promoter of E. coli MC1061 (corresponding to nucleotides 460852-463020 of the E. coli MG1655 genome^c) in pTrc99A (lacI^q and P _trc sequences deleted); Ap^R

This study

pPpiD^I350A

pPpiD carrying mutation I350 to A; Ap^R

This study

pPpiD^G347A

pPpiD carrying mutation G347 to A; Ap^R

This study

pPpiDΔ69-201

pPpiDΔ69-201; Ap^R

This study

pPpiDΔParv

pPpiDΔ252-355; Ap^R

This study

pPpiD(ΔTM)

surA signal sequence-his₆-ppiD (codons 34-623) fusion in pPpiD; Ap^R

This study

pPpiDfs601

pPpiD with a frameshift mutation in ppiD that generates a stop at codon 173; Ap^R

This study

pQE60

C-His₆ fusion vector, P _T5 /O _lac , ColEI ori; Ap^R

Qiagen

pSkp

skp gene and skp promoter of E. coli MC1061 (corresponding to nucleotides 200073-201801 of the E. coli MG1655 genome^c) in pQE60 (P_T5/O_lac deleted); Ap^R

This study

pTrc99a

Expression vector, P _trc , ColEI ori; Ap^R

Amersham Pharmacia