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Table 3 Plasmids used in this study

From: PpiD is a player in the network of periplasmic chaperones in Escherichia coli

Plasmid Genotype Source, reference
pACLacI pACYC184 derivative with lacIq; CmR This study
pASK75 vector, P/O tet , tetR, ColEI ori; ApR [60]
pASKSurAa surA gene in pASK75; ApR [2]
pASKSurAN-Ctb surAN-Ct fusion from pSurAN-Ct [2] in pASK75; ApR This study
pASKssPpiD surA signal sequence-his6-ppiD (codons 34-623) fusion in pASK75; ApR This study
pASKssPpiDΔParv pASKssPpiDΔ252-355; ApR This study
pΩSurA Ω::spec-P Llac-O1 surA in pUC18; ApR; SpecR This study
pMP1 skp gene region of E. coli MC1061 (corresponding to nucleotides 199495-201937 of the E. coli MG1655 genomec) in pSU18; CmR Gross laboratory
pPLT13 mini-F carrying lacIq; KanR [61]
pPpiD ppiD gene and promoter of E. coli MC1061 (corresponding to nucleotides 460852-463020 of the E. coli MG1655 genomec) in pTrc99A (lacIq and P trc sequences deleted); ApR This study
pPpiDI350A pPpiD carrying mutation I350 to A; ApR This study
pPpiDG347A pPpiD carrying mutation G347 to A; ApR This study
pPpiDΔ69-201 pPpiDΔ69-201; ApR This study
pPpiDΔParv pPpiDΔ252-355; ApR This study
pPpiD(ΔTM) surA signal sequence-his6-ppiD (codons 34-623) fusion in pPpiD; ApR This study
pPpiDfs601 pPpiD with a frameshift mutation in ppiD that generates a stop at codon 173; ApR This study
pQE60 C-His6 fusion vector, P T5 /O lac , ColEI ori; ApR Qiagen
pSkp skp gene and skp promoter of E. coli MC1061 (corresponding to nucleotides 200073-201801 of the E. coli MG1655 genomec) in pQE60 (PT5/Olac deleted); ApR This study
pTrc99a Expression vector, P trc , ColEI ori; ApR Amersham Pharmacia
  1. a,bReferred to as pSurA and pSurAN-Ct, respectively, in the text.
  2. caccession number NC_000913 [62]