Table 2 Strains used in this study
From: PpiD is a player in the network of periplasmic chaperones in Escherichia coli
Strain | Genotype | Source, reference, donor strain |
|---|---|---|
CAG16037 | MC1061 ϕλ[rpoH P3::lacZ] | [56] |
CAG24029 | CAG16037 surA:: Tn10d Cm | [6] |
CAG33398 | MC1061 λRS88(cpxP-lacZ) | C.A. Gross laboratory |
CAG37057 | CAG16037 Δskp zae-502:: Tn10 | C.A. Gross laboratory |
CAG44102 | MC4100 surA:: Tn10d Cm slyD1 zhg:: Tn10; CmR, TcR | [2] |
KM22 | argE3 his-4 leuB6 thr-1 ara-14 galK2 lacY1 mtl-1 xyl-5 thi-1 rpsL31 tsx-33 supE44 Δ(recC ptr recB recD):: P lac -bet exo kan | [54] |
SB10042 | CAG24029 ppiD:: Tn10 | This study; donor MC4100 ppiD:: Tn10 (T. Silhavy) |
SB11019 | CAG33398 Ω::spec-P Llac-O1 -surA pPLT13 | This study |
SB11067 | CAG33398 ppiD:: Tn10 | This study; donor MC4100 ppiD:: Tn10 (T. Silhavy) |
SB11069 | CAG33398 surA:: Tn10d Cm | This study; donor CAG24029 |
SB11072 | SB44080 ppiD::kan | This study; donor JW0431 [59] |
SB11075 | SB11069 ppiD:: Tn10 | This study; donor MC4100 ppiD:: Tn10 (T. Silhavy) |
SB11114 | CAG24029 fkpA::kan | This study; donor JW3309 [59] |
SB11116 | SB10042 fkpA::kan | This study; donor JW3309 [59] |
SB11179 | CAG33398 ppiD::kan | This study; donor JW0431 [59] |
SB44080 | CAG33398 Δskp zae-502:: Tn10 | This study; donor CAG37057 |
SB44451 | CAG37057 Ω::spec-P Llac-O1 -surA | This study |
SB44452 | CAG37057 Ω::spec-P Llac-O1 -surA pPLT13 | This study |
SB44454 | CAG16037 Ω::spec-P Llac-O1 -surA pPLT13 | This study |
SB44741 | CAG16037 ppiD:: Tn10 | This study; donor MC4100 ppiD:: Tn10 (T. Silhavy) |
SB44913 | CAG16037 ppiD::kan | This study; donor JW0431 [59] |
SB44914 | CAG37057 ppiD::kan | This study; donor JW0431 [59] |
SB44961 | SB44451 ppiD::kan pACLacI | This study; donor JW0431 [59] |
SB44964 | CAG16037 degP::kan | This study; donor JW0157 [59] |
SB44970 | SB44741 degP::kan | This study; donor JW0157 [59] |
SB44997 | CAG44080 Ω::spec-P Llac-O1 -surA pPLT13 | This study |