Extracellular Paracoccidioides brasiliensis phospholipase B involvement in alveolar macrophage interaction

BMC Microbiology

Table 3 Primers for real time RT-PCR to measure gene expression using RNA from alveolar macrophage (MH-S) cells

Cluster ID^a	Gene^b	Forward primer (5'-3')	Reverse primer (5'-3')
272294	Rps9	CGCCAGAAGCTGGGTTTGT	CGAGACGCGACTTCTCGAA
21961	nkrf	ACCTTTCAACCTACGATGGTCAGA	GAGCTCTCACATGGAATTTGGAA
575033	nfkb	AGCCAGCTTCCGTGTTTGTT	AGGGTTTCGGTTCACTAGTTTCC
104798	tnf -α	GTACCTTGTCTACTCCCAGGTTCTCT	GTGGGTGAGGAGCACGTAGTC
574821	clec 2	CTCTTCTTGGTGGCGTGTGA	AACAACCAGCCCCATGGA
3989461	il-1β	GTGTGTGACGTTCCCATTAGACA	CAGCACGAGGCTTTTTTGTTG
1346060	trl2	AAGAGGAAGCCCAAGAAAGC	CGATGGAATCGATGATGTTG
5120996	cd14	CGCAGCCTGGAATACCTTCTA	CCGCTTTAAGGACAGAGACTTGATA

^a ID: identification.
^b Abbreviations: Rps 9 (constitutive ribosomal macrophage gene); nkrf (NFKappaB repressing factor); nfkb (P50 subunit of NFKappaB); tnf-α (tumor necrosis factor-alpha); clec 2 (C- type lectin like receptor); il-1β (Interleukin-1β); trl2 (toll-like receptor 2); cd14 (glycosyl-phosphatidylinositol-anchored glycoprotein).

ISSN: 1471-2180