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Table 1 Phospholipase B activities secreted under the experimental conditions used for Phagocytic test

From: Extracellular Paracoccidioides brasiliensis phospholipase B involvement in alveolar macrophage interaction

Treatment

Specific activity of PLB

(μmol min-1mg-1protein)

Untreated control

1.21 ± 0.02

Pulmonary surfactant (100 μg mL-1)

1.55 ± 0.06* (28% activation)

Alexidine dihydrochloride (0.25 μM)

0.41 ± 0.08* (66% inhibition)

  1. Phospholipase B activities were assayed after 6 h of co-cultivation of alveolar macrophage (MH-S) cells with P. brasiliensis yeast cells with pulmonary surfactant (100 μg mL-1) and alexidine dihydrochloride (0.25 μM), as well as without treatment (untreated control), as described in Materials and Methods. *Significantly different from the untreated control, P < 0.05 by the paired 2-tailed Student's t-test. Results are means ± SEM of triplicate assays.
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BMC Microbiology

ISSN: 1471-2180

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