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Table 2 Oligonucleotide primers

From: Sialic acid transport and catabolism are cooperatively regulated by SiaR and CRP in nontypeable Haemophilus influenzae

Oligonucleotide primer Sequence1 Use
140F3 gaa ttc CTG CTT CTT CAT TAA GTT CTC GC nagA upstream
140R5 ccc ggg CAT ATT CTG TTC CTA ATA TCA ACA TCA GTT nagA upstream
140F6 ccc ggg TAA TAG TAA ACA CTT AAA TAG TTA ATT GAT TTA AAA ATC nagA downstream
140R6 gca tgc TCA AAA ACA GCA ACA CGG TGC nagA downstream
141F1 gaa ttc CAT CAT CGC TGA AAC AGG C nagB upstream
141R3 ccc ggg CAT ATT AGC CTT CCT TTA TTA TTG ACC G nagB upstream
141F3 ccc ggg TAG AGA TCT ATT CTT CAT CTT TAT GTA GGG nagB downstream
141R4 gca tgc GGT TTC AAC GCT AGT TTG GTC G nagB downstream
144F1 gaa ttc CCG TCC TTT TGT GAA TGT CC nanK upstream
144F3 ccc ggg CAT AAC TTA TCC TTA TAG TGT AAA GTC TTT TCT CAC nanK upstream
144F2 ccc ggg AAG CCT GAA GGA ACA ATT TAT GGC TAA nanK downstream
144R2 gca tgc GCC GTT TCA GCA GAA TAA CCA G nanK downstream
145F5 gaa ttc CGC TCC TGT GTG AAC TTA TG nanE upstream
145R5 ccc ggg CAT ATA ACA CCC CTC ATT TAA ATC TGA AT nanE upstream
145F6 ccc ggg TAG TCG TAA GAC GTG TGA GAA AAG ACT T nanE downstream
145R6 gca tgc CGA ACG CAA AAT CGT ATC GGC nanE downstream
604F1 gag ctc CAT TTT GCT GAC GAG GAA CTG cyaA upstream
604R1 ccc ggg CAT TAC ATA AAC ACC TAA AAT TGG TGG cyaA upstream
604F2 ccc ggg TAA TAT TTT CCT GTG GTT GAT AGG TTA CC cyaA downstream
604R2 aag ctt AAA GCA ATG GAG TGG ACC ACA ATT cyaA downstream
145R8 CCG CAG CAA TTT TTG TCC PCR SOEing
145M2 TTT ATG AAA AAA CAC TTC AAA AAT PCR SOEing
145M3 ATT TTT GAA GTG TTT TTT CAT AAA TTA AA T GTG ATC AAC TTC TC PCR SOEing
146R2 CCA TTA CGG CAC ACT AAA GAG G PCR SOEing
957F2 aag ctt AAT AAA ACG GAA TTT TTG AAA CAG G CRP expression
957R2 ctc gag TCT TGC GCC ATA TAC AAC GAT TGT CRP expression
P146F1 ACA CCC CTC ATT TAA ATC TGA ATA AAT CAC EMSA
P146R4 CCC CCA AAA TAG GAT TCG EMSA
145R7 CGA CAG GTT GGC AAG AAG AAA TAA GAC C Primer extension
145R1 ATC AGC GGC AAG AAC AGC AG Primer extension
  1. 1Restriction sites added to aid in subcloning are indicated by lowercase letters. Bases added for 5 bp insertion are italicized.