|
Strains
| | |
|
E. coli DH5α
| |
Invitrogen
|
|
E. coli BL21 Star
| |
Invitrogen
|
|
NTHi 2019
|
Clinical respiratory isolate
|
[25]
|
|
JWJ091
|
NTHi 2019ΔcyaA mutant
|
This study
|
|
JWJ093
|
NTHi 2019ΔcyaA ΔsiaR mutant, kanamycin resistant
|
This study
|
|
JWJ112
|
NTHi 2019ΔcyaA ΔnanA mutant
|
This study
|
|
JWJ114
|
NTHi 2019ΔcyaA ΔnagA mutant
|
This study
|
|
JWJ116
|
NTHi 2019ΔcyaA ΔnagB mutant
|
This study
|
|
JWJ118
|
NTHi 2019ΔcyaA ΔnanK mutant
|
This study
|
|
JWJ120
|
NTHi 2019ΔcyaA ΔnanE mutant
|
This study
|
|
JWJ159
|
NTHi 2019ΔcyaA mutant with 5 bp insertion between SiaR and Crp operators
|
This study
|
|
JWJ160
|
NTHi 2019ΔcyaA ΔnagB mutant with 5 bp insertion between SiaR and Crp operators
|
This study
|
|
Plasmids
| | |
|
pGEM-T Easy
|
PCR-cloning vector
|
Promega
|
|
pGEM-T
|
PCR-cloning vector
|
Promega
|
|
pCR2.1
|
PCR-cloning vector
|
Invitrogen
|
|
pCR2.1_443
|
pCR2.1 with PCR fragment spanning
|
This study
|
|
pUC19
|
General cloning vector
|
New England Biolabs
|
|
pET-24(+)
|
Expression vector
|
Novagen
|
|
pUC19-142del
|
nanA deletion construct
|
This study
|
|
pUC19-142sacB
|
nanA::tetR-sacB/kanR
|
This study
|
|
pJJ150
|
siaR knockout vector
|
[14]
|
|
pJJ185
|
C-terminal his-tagged SiaR expression vector
|
[14]
|
|
pJJ260
|
tetR-sacB/kanRcassette
| |
|
pJJ276
|
C-terminal his-tagged CRP expression vector
|
This study
|
|
pJJ279
|
cyaA deletion construct
|
This study
|
|
pJJ290
|
cyaA::tetR-sacB/kanR
|
This study
|
|
pJJ308
|
nagA deletion construct
|
This study
|
|
pJJ309
|
nagB deletion construct
|
This study
|
|
pJJ310
|
nanK deletion construct
|
This study
|
|
pJJ311
|
nanE deletion construct
|
This study
|
|
pJJ313
|
nagA::tetR-sacB/kanR
|
This study
|
|
pJJ314
|
nagB::tetR-sacB/kanR
|
This study
|
|
pJJ315
|
nanK::tetR-sacB/kanR
|
This study
|
|
pJJ316
|
nanE::tetR-sacB/kanR
|
This study
|
|
pJJ321
|
pCR2.1_443 with 5 bp deletion
|
This study
|
|
pJJ331
|
pJJ321 with tetR-sacB/kanR
|
This study
|
