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Table 7 Oligonucleotide primers used in this study

From: Identification of the full set of Listeria monocytogenes penicillin-binding proteins and characterization of PBPD2 (Lmo2812)

primer

Sequence 5'→3'

pET6up3a

AGCAAATCATATG GCGGTTTATTCAGTCG

pET6downa

ATGCTCGAG ATCTTCTTTAAACCCAACCTC

La2812

ATCCGCTATCTGAATCGCCT

Pb2812b

TTCAGCTGTTCCAA TTATTGCTCCGTAGAACAGGCTG

Lc2812

TTGGAACAGCTGAACGTGGA

Pd2812

CTAGAGTCAATCCGCAGCCA

La2754

CCGTTATTGACATCTGCTAC

Pb2754b

CCGCAGAAGCACCAA TAACTGCCAGCGACGTTGAA

Lc2754

TTGGTGCTTCTGCGGCTTGT

Pd2754

TAGCAGATGGCATCATCCGG

  1. aNucleotide substitutions to create restriction sites are underlined
  2. bOverhangs complementary to SOE primers are underlined
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BMC Microbiology

ISSN: 1471-2180

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