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Table 2 Competition binding assay and affinity of different PBPs of L. monocytogene s for Boc-FL

From: Identification of the full set of Listeria monocytogenes penicillin-binding proteins and characterization of PBPD2 (Lmo2812)

PBP Boc-FL Kd50a Ampicillinc
PPBA1 (PBP1) >10 μM 95
PBPB2 (PBP2) 0.25 μM 90
PBPB1 (PBP3) 0.25 μM 0
PBPA2 (PBP4) 0.25 μM 90
PBPB3 >20 μM 95
PBPD1 (PBP5) 5.0 μM 0
PBPC1 >20 μM 100
PBPC2 >20 μM 100
PBPD3 n.a. n.a.
PBPD2 2.5 μMb 0b
  1. aaffinity of the respective bands for Boc-FL estimated from their fluorescence as a function of the concentration of Boc-FL (Kd50)
  2. bobtained with purified recombinant Lmo2812
  3. cpercentage of Boc-FL binding capacity remaining after sample was preincubated with 100 μg/ml ampicillin