BMC Microbiology

Table 2 Competition binding assay and affinity of different PBPs of L. monocytogene s for Boc-FL

From: Identification of the full set of Listeria monocytogenes penicillin-binding proteins and characterization of PBPD2 (Lmo2812)

PBP	Boc-FL Kd50^a	Ampicillin^c
PPBA1 (PBP1)	>10 μM	95
PBPB2 (PBP2)	0.25 μM	90
PBPB1 (PBP3)	0.25 μM	0
PBPA2 (PBP4)	0.25 μM	90
PBPB3	>20 μM	95
PBPD1 (PBP5)	5.0 μM	0
PBPC1	>20 μM	100
PBPC2	>20 μM	100
PBPD3	n.a.	n.a.
PBPD2	2.5 μM^b	0^b

^aaffinity of the respective bands for Boc-FL estimated from their fluorescence as a function of the concentration of Boc-FL (Kd50)
^bobtained with purified recombinant Lmo2812
^cpercentage of Boc-FL binding capacity remaining after sample was preincubated with 100 μg/ml ampicillin

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