Figure 9

Differences in ROS response to mycobacterial infection between C57Bl/6 macrophages and dendritic cells. Cells were infected as described in figure 8 and ROS were detected 2 h after infection using dihydroethidium (DHE). A. BMDM or B. BMDD left untreated (UT), infected with BCG, M. smegmatis (Msme) or incubated with opsonized zymosan particles for 4 h or 24 h. The increase in DHE mediated fluorescence (FL-2) was analyzed by flow cytometry of 10,000 total cells. A representative of three independent experiments is shown.