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Figure 4 | BMC Microbiology

Figure 4

From: The non-pathogenic mycobacteria M. smegmatis and M. fortuitum induce rapid host cell apoptosis via a caspase-3 and TNF dependent pathway

Figure 4

PI-LAMs activate cells in a TLR-2-dependent manner. A. CHO/CD14/TLR-2 and B. CHO/CD14/TLR-4 reporter cell lines were incubated with the indicated lipoglycans at 20 μg/ml or LPS at 1 μg/ml for 16 h. Cellular activation was measured by determining the expression of CD25 at the cell surface by using anti-CD25 monoclonal antibodies and flow cytometry. The mean fluorescence intensities were determined and the fold induction over untreated cells was calculated and the mean and standard deviation of three independent experiments is shown.

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BMC Microbiology

ISSN: 1471-2180

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