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Figure 3 | BMC Microbiology

Figure 3

From: The non-pathogenic mycobacteria M. smegmatis and M. fortuitum induce rapid host cell apoptosis via a caspase-3 and TNF dependent pathway

Figure 3

PI-LAM of fast-growing mycobacteria induces apoptosis and IL-12 gene expression in macrophages. A. Differentiated human THP-1 cells were not treated (UT) or incubated with the indicated lipoglycans at 20 μg/ml for 24 h. The percentage of apoptotic cells was determined as Annexin-V-Alexa488-positive and propidium iodide-negative cells out of 10,000 analyzed cells by flow cytometry. B. The induction of Il-12 gene expression was analyzed by incubating a murine macrophage (RAW/pIL-12-GFP) reporter cell line which has the IL-12p40 promoter in front of the GFP gene, with the indicated lipoglycans for 16 h. GFP-expression was analyzed on 5,000 cells and the mean and standard deviation of three independent experiments is shown.

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BMC Microbiology

ISSN: 1471-2180

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