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Table 1 Genes differentially expressed in strain 13 after growth in the presence of homocysteine or cystine.

From: Global regulation of gene expression in response to cysteine availability in Clostridium perfringens

Gene name (synonym) Function/similarity Transcriptome analysis qRT-PCR  
   Homocysteine/cysteine p-value Homocysteine/cysteine  
T-box Cys controlled genes
cpe1321 (cysE) Serine acetyl-transferase 7.91 0.0001   
cpe1322 (cysK) OAS-thiol-lyase 6.86 0.0002 120  
cpe0967 Na+-H+/Amino acid symporter 15.53 6.6E-06   
cpe0947 Na+-H+/Amino acid symporter 7.01 0.0002   
S-box controlled genes
cpe2177 (metK) SAM-synthase 2.7 0.015 14  
cpe2317 probable Na+-H+ antiporter 1.4 0.01   
Iron sulfur clusters
cpe1786 Rrf2-type regulator 3.41 0.0001 14  
cpe1785 (iscS) Cysteine desulfurase 3.36 0.00027   
cpe1784 (iscU) Iron sulfur cluster assembly 6.73 0.00008   
cpe1783 (trmU) Methylaminomethyl-2-Thiouridylate- 3.5 < 1E-05   
  methyltransferase     
cpe1469 IscS-like protein 2.5 0.0009 8  
cpe0664 HesB-like protein 3.83 1.5E-05 11  
Functions associated to redox
cpe2511 (fer) Ferredoxin [3Fe-4S] 3.2 < 1E-05   
cpe777 (rubR1) Rubredoxin 1.8 0.001   
cpe0780 (rubR2) Rubredoxin 2.4 < 1E-05 4.3  
cpe0778 Probable flavohemoprotein 1.62 0.005   
cpe1331 (rubY) Rubrerythrine 1.64 0.01   
cpe 2447 (fer) Ferredoxin 2[2Fe-2S] 0.52 0.01   
cpe0782 Alkyl hydrogen peroxide reductase 0.49 < 1E-05   
cpe 2537 cytochrome c-type biogenesis protein 0.41 < 1E-05   
cpe 2538 Unknown 0.25 3.5E-05   
Carbon metabolism
cpe2308 Mannose-1-phosphate 3.5 2.3E-05   
  guanylyltransferase     
cpe0103 (ldh) Lactate dehydrogenase 2.73 0.004 15  
Transporters, membrane or exported proteins
cpe2151 Mercure-copper binding protein 5.1 < 1E-05   
cpe1371 Na+-dependent symporter 3.3 0.009 5  
cpe0049 Membrane protein 3.02 < 1E-05   
cpe2456 Membrane protein 2.84 1E-05   
cpe0554 Protein with signal sequence 2.74 0.0002   
cpe0383 Holin-like protein 2.6 0.004   
cpe2595 Na+/H+ antiporter 0.34 < 1E-05   
Virulence
cpe0163 Perfringolysin O 0.3 0.02 0.16  
cpe1523 (nagL) Hyaluronidase 1.82 9.5E-05 2.3  
Proteins of unknown function
cpe1078 Unknown (73 aa) 10.8 < 1E-05   
cpe1079 Unknown 7.77 3E-05   
cpe1385 Unknown 4.2 6E-05   
cpe1386 Unknown (85aa) 8.73 < 1E-05   
cpe1387 Unknown (71aa) 6.4 5E-06   
cpe1388 Unknown 5.94 5E-05   
cpe0651 Unknown 4.8 < 1E-05   
cpe0015 Unknown 4.7 2E-05   
cpe0114 Unknown (74 aa) 4.49 1.33E-05   
cpe0113 Unknown (75 aa) 3.98 6E-05   
cpe0264 Unknown (98 aa) 3.94 0.001   
cpe2619 Unknown (63 aa) 3.88 < 1E-05   
cpe0067 Unknown 3.6 1E-05   
cpe0102 Unknown (90 aa) 3.52 0.01   
cpe1472 Unknown 3.36 0.00735   
cpe2037 Unknown (89 aa) 3.16 0.0006   
cpe0363 Unknown (118 aa) 3.11 0.002   
  1. The results presented are representative of 8 differential hybridizations using RNA preparations obtained from 4 independent cultures. aa means amino acids.