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Table 1 Experimental overview

From: Sub-chronic lung inflammation after airway exposures to Bacillus thuringiensis biopesticides in mice

Exp.No.

Aim of experiment

Number of mice

Exposure method

Substance

Time Endpoint

Endpoint

Corresponding

figure

1

Validation of Inhalation dose

10

Inhalation (1 hour)

Vectobac®

1 h

CFU from total lung homogenate

Figure 1

2

Validation of CFU recovery from BALF

8

Inhalation

Vectobac®

1 h

CFU from BALF and lavaged lungtissue

None

3

Dose- response relationship B.t israelensis

25

Instillation

Vectobac®

24 h

Inflammatory cells in BALF

Figure 2

4

Time- response relationship B.t israelensis or B.t kurstaki

42

Instillation

Vectobac® or Dipel® (washed)

4 h, 24 h, 4 days

CFU and inflammatory cells in BALF

Figure 3

5

Sub-chronic effects of i.t instillations of B.t israelensis or B.t kurstaki

20

Instillation

Vectobac® or Dipel®

70 days

CFU, Inflammatory cells in BALF, Histology

Figure 4

Figure 5

6

Sub-chronic effects of repeated inhalations of B.t israelensis or B.t kurstaki

18

Inhalation (Repeated)

Vectobac® or Dipel®

70 days

Airway irritation, CFU, Inflammatory cells in BALF, Histology

Figure 5

  1. Mice were exposed to Bt israelensis (Vectobac®) or Bt kurstaki (Dipel®) by either intratracheal instillation or inhalation of bacterial suspension. At 4 hours (h), 24 h, 4 days or 70 days after exposure, lungs were lavaged and the bronchoalveolar lavage fluid (BALF) was analysed for content of colony forming units (CFU) and inflammatory cells. Furthermore, histological examination of the lung tissue was performed where specified.