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Table 1 Experimental overview

From: Sub-chronic lung inflammation after airway exposures to Bacillus thuringiensis biopesticides in mice

Exp.No. Aim of experiment Number of mice Exposure method Substance Time Endpoint Endpoint Corresponding
figure
1 Validation of Inhalation dose 10 Inhalation (1 hour) Vectobac® 1 h CFU from total lung homogenate Figure 1
2 Validation of CFU recovery from BALF 8 Inhalation Vectobac® 1 h CFU from BALF and lavaged lungtissue None
3 Dose- response relationship B.t israelensis 25 Instillation Vectobac® 24 h Inflammatory cells in BALF Figure 2
4 Time- response relationship B.t israelensis or B.t kurstaki 42 Instillation Vectobac® or Dipel® (washed) 4 h, 24 h, 4 days CFU and inflammatory cells in BALF Figure 3
5 Sub-chronic effects of i.t instillations of B.t israelensis or B.t kurstaki 20 Instillation Vectobac® or Dipel® 70 days CFU, Inflammatory cells in BALF, Histology Figure 4
Figure 5
6 Sub-chronic effects of repeated inhalations of B.t israelensis or B.t kurstaki 18 Inhalation (Repeated) Vectobac® or Dipel® 70 days Airway irritation, CFU, Inflammatory cells in BALF, Histology Figure 5
  1. Mice were exposed to Bt israelensis (Vectobac®) or Bt kurstaki (Dipel®) by either intratracheal instillation or inhalation of bacterial suspension. At 4 hours (h), 24 h, 4 days or 70 days after exposure, lungs were lavaged and the bronchoalveolar lavage fluid (BALF) was analysed for content of colony forming units (CFU) and inflammatory cells. Furthermore, histological examination of the lung tissue was performed where specified.