From: Sub-chronic lung inflammation after airway exposures to Bacillus thuringiensis biopesticides in mice
Exp.No. | Aim of experiment | Number of mice | Exposure method | Substance | Time Endpoint | Endpoint | Corresponding figure |
---|---|---|---|---|---|---|---|
1 | Validation of Inhalation dose | 10 | Inhalation (1 hour) | Vectobac® | 1 h | CFU from total lung homogenate | Figure 1 |
2 | Validation of CFU recovery from BALF | 8 | Inhalation | Vectobac® | 1 h | CFU from BALF and lavaged lungtissue | None |
3 | Dose- response relationship B.t israelensis | 25 | Instillation | Vectobac® | 24 h | Inflammatory cells in BALF | Figure 2 |
4 | Time- response relationship B.t israelensis or B.t kurstaki | 42 | Instillation | Vectobac® or Dipel® (washed) | 4 h, 24 h, 4 days | CFU and inflammatory cells in BALF | Figure 3 |
5 | Sub-chronic effects of i.t instillations of B.t israelensis or B.t kurstaki | 20 | Instillation | Vectobac® or Dipel® | 70 days | CFU, Inflammatory cells in BALF, Histology | Figure 4 Figure 5 |
6 | Sub-chronic effects of repeated inhalations of B.t israelensis or B.t kurstaki | 18 | Inhalation (Repeated) | Vectobac® or Dipel® | 70 days | Airway irritation, CFU, Inflammatory cells in BALF, Histology | Figure 5 |