Table 2 Sequences of primers used in co-transcription RT-PCR assays, cloning experiments and EMSA assays.
Primers used in RT-PCR assays | |||||
|---|---|---|---|---|---|
cbb1 operon | |||||
Numbera | Gene | Forward primer (5'-3') | Numbera | Gene | Reverse primer (5'-3') |
1 | cbbR | CAACGCCGTGTTGCTCGAA | 2 | cbbL1 | CTAGACTTTTTTACGGCCATGCTT |
3 | cbbL1 | CTGCCAATCGTGTCGCGC | 4 | csoS2 | CGCACGGGAAAGCGACTT |
5 | csoS2 | CCTATGGTGCCGTGCCAAC | 6 | csoS3 | GTGCATGACGCACGCCC |
7 | csoS3 | GTCAGCGGGTCAAAGCCG | 8 | csoS1A | GCCGCCTTGGTCATCG |
9 | csoS1B | GGAGCAGATGCGTGTGAGCG | 11 | parA | AGTAGAACCCCGCCGAGCCAA |
10 | bfrA | CGCGCAGAAGAGTTACAAGCCTTG | 12 | parA | CTGATCGAACCCTGAGGATCGG |
13 | parA | GTGCTGCGGTTGAAGGGGT | 14 | hyp2 | GTGGAGTTCGATAATGGCGGAG |
15 | hyp2 | CGAGAAGCCTCCGCCATTATC | 16 | cbbQ1 | GCCTGTGGGTCCTTTCAGCAT |
18 | cbbO1 | TGACGCCAGGAAAGCGGTG | 17 | cbbO1 | CAGGGATTTCAGGCTGGTCG |
19 | cbbO1 | GCAGAGGCTGCCAGAAAAGCT | 20 | cbbA | AAGCACCTACCGCGTATCCGT |
21 | bioD a | CAGTGCCACCGCCACCC | |||
cbb2 operon | |||||
Forward primer (5'-3')' | Reverse primer (5'-3') | ||||
1 | tatC a | ACGACGGCGTCTAGAACCGCC | 2 | cbbL2 | CCGGTAATCCTCTAGACCCGCGTT |
3 | cbbL2 | CATCGAGAAGGAAGGCAAGGC | 4 | cbbS2 | CGCAACCTGTTGACGGATCTG |
5 | cbbS2 | ACCGGAAAACGCCTTCGGC | 6 | cbbQ2 | GGTCAATGGGCCATCCTGCC |
7 | cbbQ2 | AGGGTGTTGAGGCGAAGGCC | 8 | cbbO2 | GTACGATGGGCGTGTGCGC |
9 | cbbO2 | GCCTACAGCGAGGAGGCCATG | 10 | yfeA a | GCGGAGCCTTGTCCCTCGG |
cbb3 operon | |||||
Forward primer (5'-3')' | Reverse primer (5'-3') | ||||
1 | hyp4 a | TACGAAGGCGGCTCCCCG | 2 | hyp3 | CGACGGCAATCGGAGTCTTT |
3 | hyp3 | CGGGTGATCGCGCTGGAT | 4 | cbbT | CAGAATGCCGTCGTGACCA |
5 | cbbT | ATCGGCATCGACCACTTT | 6 | cbbK | TCCATCATACGCAGGACA |
7 | cbbK | CCTACATCAGTACGGGTG | 9 | cbbA | CACCTGCTCCAGGTTGTT |
8 | pykA | TTGATCCTCATCACCATCGG | 11 | cbbE | GATATGGATATAGTCGGCACCC |
10 | cbbA | GCAGGCCAGCAAGATCAA | 14 | trpE | GCCGACAAGGGAGTATCGA |
12 | cbbE | CTATCGAACTGGAAGTGGATGG | 16 | trpG | CGATAGCCGCCACGTCG |
13 | cbbZ | TCGGCGATTCACGTAACG | 17 | trpC a | AGGGCCACTGCCGCCTGC |
15 | trpE | GAAACCATGAACAAACGCCG | |||
cbb4 operon | |||||
Forward primer (5'-3')' | Reverse primer (5'-3') | ||||
1 | ompA a | GGTATTTCCTATTTTTGGGGTGGC | 3 | sahA | CGGCAATGCGGACTTCCTTAC |
2 | metK | TTGGGAGCGGACCGACAAG | 5 | metF | AAGCATACTCGGGACCCAAGG |
4 | sahA | CTTCGCGGGGGTGCTGA | 7 | cbbP | GACGGGATGTTTTTTGGACATGG |
6 | metF | CACCGAGCCTGCATTTTTACACC | 9 | ynbD | GGCTACAGCCACCACGGGAT |
8 | cbbP | ATGTTGCCGGGCAGTTTTATGTC | 10 | fbnA a | GCGAGGTGGACTGGACGGA |
Primers used in EMSA assays and cloning experiments | |||||
Letter designation | Forward primer (5'-3') | Reverse primer (5'-3') | |||
(a) Pcbb1 | CGGCAGCGAAGATCTTGAGTTGGTGC | (b) Pcbb1 | CTCCGGCCTCATACTTTTTTACGG | ||
(c) cbbRfw | TCTATCCGTCATGCAACCTTG | (d) cbbRrev | GCGCCATTCCTTTTCACCATG | ||
(e) Pcbb2 | ACGACGGCGGCAAGCACCGCC | (f) Pcbb2 | CCGGTAATCCTTCACACCCGCGTT | ||
(g) Pcbb3 | CATTGAACAGGGTCAGCTCCTGG | (h) Pcbb3 | ATCGGAGTCTTTGATCATGCGCC | ||
(i) Pcbb4 | TTTGGGGTGGCAGCAAGAAGT | (j) Pcbb4 | GGAAACGGATTCAGAGGTGAAAAG | ||