Primers used in RT-PCR assays
|
---|
cbb1 operon
|
---|
Numbera
|
Gene
|
Forward primer (5'-3')
|
Numbera
|
Gene
|
Reverse primer (5'-3')
|
---|
1
|
cbbR
|
CAACGCCGTGTTGCTCGAA
|
2
|
cbbL1
|
CTAGACTTTTTTACGGCCATGCTT
|
3
|
cbbL1
|
CTGCCAATCGTGTCGCGC
|
4
|
csoS2
|
CGCACGGGAAAGCGACTT
|
5
|
csoS2
|
CCTATGGTGCCGTGCCAAC
|
6
|
csoS3
|
GTGCATGACGCACGCCC
|
7
|
csoS3
|
GTCAGCGGGTCAAAGCCG
|
8
|
csoS1A
|
GCCGCCTTGGTCATCG
|
9
|
csoS1B
|
GGAGCAGATGCGTGTGAGCG
|
11
|
parA
|
AGTAGAACCCCGCCGAGCCAA
|
10
|
bfrA
|
CGCGCAGAAGAGTTACAAGCCTTG
|
12
|
parA
|
CTGATCGAACCCTGAGGATCGG
|
13
|
parA
|
GTGCTGCGGTTGAAGGGGT
|
14
|
hyp2
|
GTGGAGTTCGATAATGGCGGAG
|
15
|
hyp2
|
CGAGAAGCCTCCGCCATTATC
|
16
|
cbbQ1
|
GCCTGTGGGTCCTTTCAGCAT
|
18
|
cbbO1
|
TGACGCCAGGAAAGCGGTG
|
17
|
cbbO1
|
CAGGGATTTCAGGCTGGTCG
|
19
|
cbbO1
|
GCAGAGGCTGCCAGAAAAGCT
|
20
|
cbbA
|
AAGCACCTACCGCGTATCCGT
|
| | |
21
|
bioD
a
|
CAGTGCCACCGCCACCC
|
cbb2
operon
|
|
Forward primer (5'-3')'
| |
Reverse primer (5'-3')
| |
1
|
tatC
a
|
ACGACGGCGTCTAGAACCGCC
|
2
|
cbbL2
|
CCGGTAATCCTCTAGACCCGCGTT
|
3
|
cbbL2
|
CATCGAGAAGGAAGGCAAGGC
|
4
|
cbbS2
|
CGCAACCTGTTGACGGATCTG
|
5
|
cbbS2
|
ACCGGAAAACGCCTTCGGC
|
6
|
cbbQ2
|
GGTCAATGGGCCATCCTGCC
|
7
|
cbbQ2
|
AGGGTGTTGAGGCGAAGGCC
|
8
|
cbbO2
|
GTACGATGGGCGTGTGCGC
|
9
|
cbbO2
|
GCCTACAGCGAGGAGGCCATG
|
10
|
yfeA
a
|
GCGGAGCCTTGTCCCTCGG
|
cbb3
operon
|
| |
Forward primer (5'-3')'
| | |
Reverse primer (5'-3')
|
1
|
hyp4
a
|
TACGAAGGCGGCTCCCCG
|
2
|
hyp3
|
CGACGGCAATCGGAGTCTTT
|
3
|
hyp3
|
CGGGTGATCGCGCTGGAT
|
4
|
cbbT
|
CAGAATGCCGTCGTGACCA
|
5
|
cbbT
|
ATCGGCATCGACCACTTT
|
6
|
cbbK
|
TCCATCATACGCAGGACA
|
7
|
cbbK
|
CCTACATCAGTACGGGTG
|
9
|
cbbA
|
CACCTGCTCCAGGTTGTT
|
8
|
pykA
|
TTGATCCTCATCACCATCGG
|
11
|
cbbE
|
GATATGGATATAGTCGGCACCC
|
10
|
cbbA
|
GCAGGCCAGCAAGATCAA
|
14
|
trpE
|
GCCGACAAGGGAGTATCGA
|
12
|
cbbE
|
CTATCGAACTGGAAGTGGATGG
|
16
|
trpG
|
CGATAGCCGCCACGTCG
|
13
|
cbbZ
|
TCGGCGATTCACGTAACG
|
17
|
trpC
a
|
AGGGCCACTGCCGCCTGC
|
15
|
trpE
|
GAAACCATGAACAAACGCCG
| | | |
cbb4
operon
|
| |
Forward primer (5'-3')'
| | |
Reverse primer (5'-3')
|
1
|
ompA
a
|
GGTATTTCCTATTTTTGGGGTGGC
|
3
|
sahA
|
CGGCAATGCGGACTTCCTTAC
|
2
|
metK
|
TTGGGAGCGGACCGACAAG
|
5
|
metF
|
AAGCATACTCGGGACCCAAGG
|
4
|
sahA
|
CTTCGCGGGGGTGCTGA
|
7
|
cbbP
|
GACGGGATGTTTTTTGGACATGG
|
6
|
metF
|
CACCGAGCCTGCATTTTTACACC
|
9
|
ynbD
|
GGCTACAGCCACCACGGGAT
|
8
|
cbbP
|
ATGTTGCCGGGCAGTTTTATGTC
|
10
|
fbnA
a
|
GCGAGGTGGACTGGACGGA
|
Primers used in EMSA assays and cloning experiments
|
Letter designation
|
Forward primer (5'-3')
| |
Reverse primer (5'-3')
|
(a) Pcbb1
|
CGGCAGCGAAGATCTTGAGTTGGTGC
|
(b) Pcbb1
|
CTCCGGCCTCATACTTTTTTACGG
|
(c) cbbRfw
|
TCTATCCGTCATGCAACCTTG
|
(d) cbbRrev
|
GCGCCATTCCTTTTCACCATG
|
(e) Pcbb2
|
ACGACGGCGGCAAGCACCGCC
|
(f) Pcbb2
|
CCGGTAATCCTTCACACCCGCGTT
|
(g) Pcbb3
|
CATTGAACAGGGTCAGCTCCTGG
|
(h) Pcbb3
|
ATCGGAGTCTTTGATCATGCGCC
|
(i) Pcbb4
|
TTTGGGGTGGCAGCAAGAAGT
|
(j) Pcbb4
|
GGAAACGGATTCAGAGGTGAAAAG
|
- aGenes lie outside the operons and are not shown in Figure 2.