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Table 1 List of bacterial strains and plasmids used in this study

From: Genes and pathways for CO2 fixation in the obligate, chemolithoautotrophic acidophile, Acidithiobacillus ferrooxidans, Carbon fixation in A. ferrooxidans

Strain or plasmid Relevant characteristic Source or reference
Bacterial strains   
Type strain ATCC 23270
E. coli TOP10 F- mcr A Δ(mrr-hsd RMS-mcr BC) ϕ80lac ZΔM15 Δlac X74 rec A1 ara D139 Δ(ara-leu) 7697 gal U gal K rps L (StrR) end A1 nup G Invitrogen
pBAD-TOPO® AmpR promoter ara BAD (PBAD) C-terminal: V5 epitope tag-polyhistidine (6 × His) Invitrogen
pBAD-cbb R pBAD-TOPO::927-bp fragment containing cbb R from A. ferrooxidans ATCC 23270 expressed from PBAD promoter This study
  1. Abbreviations used: ATCC, American Type Culture Collection. AmpR, ampicillin resistance; StrR, streptomycin resistance.