Table 3 Primers used in this study
Primer | Primer sequence 5'-3' | RE site |
|---|---|---|
pilA LFF | GAGCTCACGCGT-CTTACTTGCCGGATCATTACCAAC | SphI |
pilA LFR | CTGCAG-CCTTCTTTATAGTTTAGTTTAC | PstI |
pilA RFF | CTGCAGGTAGATAAACTAAGCCACTTTCATGTG | PstI |
pilA RFR | GGATCCGCATGCTCAAGGCTTCTGTCAATCTTGTTC | MluI |
CAM PstIF | GCCTGCAGGTAAGAGGTTCCAACTTTCAC | PstI |
CAM PstIR | TGATCTGCAGTTACGCCCCGCCCTGCCACTCATC | PstI |
PilC-A | GCATGTCCTAGGGTCAAGCTTAGATATTGCTGAA | AvrII |
PilC-B | TATATCGCATCGCCAAATAGCATATTTTTTATTCC | |
PilC-C | GCTATTTGGCGATGCGATATAATATACTTTTAAAAA | |
PilC-D | GCATGTGTCGACGTCCTGAGAAAATATCTAGACA | SalI |
PilT-A | CATTATGTCGACTATGCAACAGTTCTTGATGGT | SalI |
PilT-B | TACTACAATGTATAGTAATTTTCTTATCATATCAAG | |
PilT-C | AGAAAATTACTATACATTGTAGTAAGGTAATCA | |
PilT-D | CATTATTCTAGACAGGATTAACGGCAGCTAAAA | XbaI |
PilQ-A3 | GCATGTCCTAGG TCAGTCAATGGAAGCACAGAT | AvrII |
PilQ-B3 | TATCTGCTATCATGTTAGAACAACTAATAACTTCTT | |
PilQ-C3 | TTGT TCTAACATGATAGCAGATAATAGTTGCAAA | |
PilQ-D3 | GCATGTGTCGACAGAAAGTAATGTTGTTGGTATTT | SalI |
RT-PCR primers | ||
PilA_A | GATCCCGATGTACTCTAACTA | |
PilA_B | CCATTAGCTCAACTAGTGAGAA | |
PilA_C | ATCTTAGCAGCTGTAGCAATA | |
PilA_D | GGGGTAGTACTTTAAATCCT | |
PilA_E | CTTACTGAGTTACTTGTTGTTAT | |
PilA_F | GTCTTTCTGATCTATATGCTTC | |
PilC_A | GTCAAGCTTAGATATTGCTGAA | |
PilC_B | GTCTCTGGAGCACTGTTTGTAT | |
PilC_C | AAGGTAGTATTGATGCTGACAC | |
PilC_D | CCGTTGCTAAAGACACCATA | |
PilC_E | GATGCGATATAATATACTTTTAAAAA | |
PilC_F | CGAATTGGTATTGGCCAGAT | |
PilQ_A | TATGGTCAGGTAGAAGATGTAA | |
PilQ_B | CATCAATTTACCTTACTAATGTAT | |
PilQ_C | GCCTGAGCAGTAGTATAGTTT | |
PilQ_D | AGTTGGTGCTGGAAAATCTAC | |
PilQ_E | CAGGATAGTTTCTTCTACTAAA | |
PilT_A | CTATTAGGCGTGAAAGCAGTT | |
PilT_B | TAGTAATTTTCTTATCATATCAAG | |
PilT_C | ATGATGCGAGATTTAGGGTA | |
PilT_D | CAGCAGGTGGAAATACAGAT | |
PilT_E | TACATTGTAGTAAGGTAATCA | |
PilT_F | GGTAGAGTTGAATCAGCGTTTA |