| Description, characteristicsa or sequence (5'→3') forward primer, reverse primer | Source or reference |
---|---|---|
Plasmid | Â | Â |
pAS222 | Shuttle vector, TetR | [25] |
pGhostΔmpD | Insertion inactivation vector of mptD | This work |
Strain | Â | Â |
E. coli EPI300 | Â | Epicentre Technologies, USA |
E. faecalis V583 | Wild type | [20] |
MOP1 | Resistant mutant, from exposure to pediocin PA-1 10 BU/ml | This work |
MOP2 | Resistant mutant, from exposure to 10 mM 2-deoxsyglucose | This work |
MOP5 | Resistant mutant, from exposure to pediocin PA-1 640 BU/ml | This work |
MOM1 | Inserted inactivated mptD | This work |
Pediococcus acidilactici Pac 1.0 | Pedioicn PA-1 producer | [21] |
Primer | Â | Target DNA |
arcA-F | TAACTCGACAACGGGAAACC | EF0104, arcA |
arcA-R | TCCCAATGGCCACTACTTCT | EF0104, arcA |
citE-F | CGGTGATTAACCCTCGTCAA | EF3320, citE |
citE-R | ACGGAGATAACACCGGAACC | EF3320, citE |
dnaB-F | TAGAAATGGGGGCAGAATCA | EF0013, dnaB |
dnaB-R | ATTCGCACGGGACAAACTAC | EF0013, dnaB |
mptAB-F | TGACCTATGGGGAGGAACAC | EF0020, mptAB |
mptAB-R | GTCGCAATTTCTTGTGCTGA | EF0020, mptAB |
mptC-F | ATTCGTATTGCGATTCCAGCA | EF0021, mptC |
mptC-R | TGCATAACCTACGGCAACGAC | EF0021, mptC |
mptD-F | TCGTTGGTCATTCATGTGGT | EF0022, mptD |
mptD-R | GTTGAACTAATGCGGCCAGT | EF0022, mptD |
mptDi-F | GAAGGAGGAGCAAAGAAAATGGCA | EF0022, mptD |
mptDi-R | CACCGACACCGGCTAAAGGAC | EF0022, mptD |
mptO-F | TATCCAAATTCCGTGGGAAG | EF0024, manO |
mptO-R | TAACACTCGCTTCGGCTCTT | EF0024, manO |
pgk-F | AATGACGCTCCTTTCCACAC | EF1963, pgk |
pgk-R | TTTCAAATACGCCCATTGGT | EF1963, pgk |