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Table 1 Plasmids, bacterial strains and primers used in this study

From: Class IIa bacteriocin resistance in Enterococcus faecalis V583: The mannose PTS operon mediates global transcriptional responses

 

Description, characteristicsa or sequence (5'→3') forward primer, reverse primer

Source or reference

Plasmid

  

pAS222

Shuttle vector, TetR

[25]

pGhostΔmpD

Insertion inactivation vector of mptD

This work

Strain

  

E. coli EPI300

 

Epicentre Technologies, USA

E. faecalis V583

Wild type

[20]

MOP1

Resistant mutant, from exposure to pediocin PA-1 10 BU/ml

This work

MOP2

Resistant mutant, from exposure to 10 mM 2-deoxsyglucose

This work

MOP5

Resistant mutant, from exposure to pediocin PA-1 640 BU/ml

This work

MOM1

Inserted inactivated mptD

This work

Pediococcus acidilactici Pac 1.0

Pedioicn PA-1 producer

[21]

Primer

 

Target DNA

arcA-F

TAACTCGACAACGGGAAACC

EF0104, arcA

arcA-R

TCCCAATGGCCACTACTTCT

EF0104, arcA

citE-F

CGGTGATTAACCCTCGTCAA

EF3320, citE

citE-R

ACGGAGATAACACCGGAACC

EF3320, citE

dnaB-F

TAGAAATGGGGGCAGAATCA

EF0013, dnaB

dnaB-R

ATTCGCACGGGACAAACTAC

EF0013, dnaB

mptAB-F

TGACCTATGGGGAGGAACAC

EF0020, mptAB

mptAB-R

GTCGCAATTTCTTGTGCTGA

EF0020, mptAB

mptC-F

ATTCGTATTGCGATTCCAGCA

EF0021, mptC

mptC-R

TGCATAACCTACGGCAACGAC

EF0021, mptC

mptD-F

TCGTTGGTCATTCATGTGGT

EF0022, mptD

mptD-R

GTTGAACTAATGCGGCCAGT

EF0022, mptD

mptDi-F

GAAGGAGGAGCAAAGAAAATGGCA

EF0022, mptD

mptDi-R

CACCGACACCGGCTAAAGGAC

EF0022, mptD

mptO-F

TATCCAAATTCCGTGGGAAG

EF0024, manO

mptO-R

TAACACTCGCTTCGGCTCTT

EF0024, manO

pgk-F

AATGACGCTCCTTTCCACAC

EF1963, pgk

pgk-R

TTTCAAATACGCCCATTGGT

EF1963, pgk

  1. aTetR, tetracycline resistance