Table 2 Results of pncA gene sequencing of 150 M. tuberculosis clinical isolates.
M. tuberculosis strains (no. of isolates) | MGIT 960 | PZase assay | pncA mutation | |
|---|---|---|---|---|
| Â | Â | Â | Nucleotide change | Amino acid change |
Susceptible (46) | S | + | wild-type | no |
Susceptible (1) | S | + | T92G | Ile31Ser |
Susceptible (2) | R | + | wild-type | wild-type |
Susceptible (1) | R | + | T92C | Ile31Thr |
MDR-TB (42) | S | + | wild-type | wild-type |
MDR-TB (9) | S | + | T92C | Ile31Thr |
MDR-TB (34) | R | - | A(-11)G (1) | no |
| Â | Â | Â | A(-11)C (1) | no |
| Â | Â | Â | T56G (1) | Leu19Arg |
| Â | Â | Â | T80C (1) | Leu27Pro |
| Â | Â | Â | T92G (2) | Ile31Ser |
| Â | Â | Â | T104C (1) | Leu35Pro |
| Â | Â | Â | T134C (1) | Val45Ala |
| Â | Â | Â | G136T (1) | Ala46Ser |
| Â | Â | Â | T199C (1) | Ser67Pro |
| Â | Â | Â | C211G (8) | His71Asp |
| Â | Â | Â | G215A (1) | Cys72Tyr |
| Â | Â | Â | G222C (1) | Gly74Arg |
| Â | Â | Â | G289A (3) | Gly97Ser |
| Â | Â | Â | C312G (2) | Ser104Arg |
| Â | Â | Â | G364A (1) | Gly122Ser |
| Â | Â | Â | G373T (1) | Val125Phe |
| Â | Â | Â | G379T (1) | Glu 127 Stop |
| Â | Â | Â | G insertion b/w 411-412 (1) | Â |
| Â | Â | Â | T416G (1) | Val 139 Gly |
| Â | Â | Â | C425T (1) | Thr 142 Met |
| Â | Â | Â | G436A (1) | Ala 146 Thr |
| Â | Â | Â | C520T (1) | Thr 174 Ile |
| Â | Â | Â | GG insertion b/w 520-521 (1) | Â |
MDR-TB (11) | R | + | wild-type | no |
MDR-TB (4) | R | + | T92C (3) | Ile31Thr |
| Â | Â | Â | T92G (1) | Ile31Ser |