Table 2 Properties of R. leguminosarum wildtype and flagellin mutants

Strain 3841

ABCDEHG

Normal (4.7 ± 0.5um; n = 8)

3841 flaA^-

BCDEHG

Almost all cells are non-flagellated; only one cell with very thin, short appendage

3841 flaB^-

ACDEHG

Truncated (2.2 ± 0.5um; n = 6)

3841 flaC^-

ABDEHG

ND

3841 flaD^-

ABCEHG

ND

3841 flaE^-

ABCDHG

Truncated (3.4 ± 0.3 um; n = 5)

3841 flaH^-

ABCDEG

Truncated (2.4 ± 0.6 um; n = 12)

3841 flaG^-

ABCDEH

ND

3841 flaB/C/D^-

AEHG

Truncated (1.9 ± 0.6 um; n = 13)

3841 flaA/B/C/D^-

EHG

ND

Strain VF39SM

ABCDEHG

Normal (5.1 ± 0.5 um; n = 13)

VF39SM flaA^-

BCDEHG

No flagella

VF39SM flaB^-

ACDEHG

Truncated (1.6 ± 0.5 um; n = 6); reduced number of filaments (1-2 filaments/cell)

VF39SM flaC^-

ABDEHG

Truncated (2.1 ± 0.5 um; n = 9); reduced number of filaments (1-2 filaments/cell)

VF39SM flaD^-

ABCEHG

Normal number and length; thinner filaments

VF39SM flaE^-

ABCDHG

Normal

VF39SM flaH^-

ABCDEG

Normal

VF39SM flaG^-

ABCDEH

Normal; slightly reduced number of filaments

VF39SM flaB/C/D^-

AEHG

Truncated (1.6 ± 0.3 um; n = 13); reduced number of filaments (1-2 filaments/cell)

VF39SM flaA/B/C/D^-

EHG

No flagella