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Table 2 Properties of R. leguminosarum wildtype and flagellin mutants

From: Characterization and functional analysis of seven flagellin genes in Rhizobium leguminosarum bv. viciae. Characterization of R. leguminosarum flagellins

Strain/Mutant Effective Fla subunit(s) Swimming diameter* Swarming ability* Filament Morphology†
Strain 3841 ABCDEHG 100 +++ Normal (4.7 ± 0.5um; n = 8)
3841 flaA- BCDEHG 8 - Almost all cells are non-flagellated; only one cell with very thin, short appendage
3841 flaB- ACDEHG 47 + Truncated (2.2 ± 0.5um; n = 6)
3841 flaC- ABDEHG 30 ++ ND
3841 flaD- ABCEHG 87 +++ ND
3841 flaE- ABCDHG 39 ++++ Truncated (3.4 ± 0.3 um; n = 5)
3841 flaH- ABCDEG 54 +++ Truncated (2.4 ± 0.6 um; n = 12)
3841 flaG- ABCDEH 96 ++ ND
3841 flaB/C/D- AEHG 26 + Truncated (1.9 ± 0.6 um; n = 13)
3841 flaA/B/C/D- EHG - - ND
Strain VF39SM ABCDEHG 100 +++++ Normal (5.1 ± 0.5 um; n = 13)
VF39SM flaA- BCDEHG - - No flagella
VF39SM flaB- ACDEHG 41 ++ Truncated (1.6 ± 0.5 um; n = 6); reduced number of filaments (1-2 filaments/cell)
VF39SM flaC- ABDEHG 49 ++ Truncated (2.1 ± 0.5 um; n = 9); reduced number of filaments (1-2 filaments/cell)
VF39SM flaD- ABCEHG 85 ++++ Normal number and length; thinner filaments
VF39SM flaE- ABCDHG 92 ++++ Normal
VF39SM flaH- ABCDEG 97 +++++ Normal
VF39SM flaG- ABCDEH 100 +++ Normal; slightly reduced number of filaments
VF39SM flaB/C/D- AEHG 25 + Truncated (1.6 ± 0.3 um; n = 13); reduced number of filaments (1-2 filaments/cell)
VF39SM flaA/B/C/D- EHG - - No flagella
  1. *Percentage relative to wildtype swimming diameter. Means of at least two replicates. (-) means non-motile; † As observed by TEM; ND means not determined; values in parenthesis refer to the average length of a flagellar filament ± standard deviation. The lengths of the flagella formed by the fla mutants are significantly different from the flagella formed by the wildtype strain (P < 0.0001).