Glycoprotein staining of R. leguminosarum flagellin proteins. A. Pro-Q Emerald 300 stain. Lane 1-Molecular marker. Molecular masses (in kDa) are shown on the left of panel B; Lane 2-CandyCane glycoprotein molecular weight standard, 42kDa α1-Acid glycoprotein served as a positive control (shown in panel A) and a 29kDa-protein, carbonic anhydrase (shown in panel B) served as a negative control for glycosylation; Lane 3 - VF39SM; Lane 4 - 3841. B. Coomassie Brilliant Blue stain to demonstrate total proteins. Same sample arrangement as in panel A.