Matrix metalloproteinase-3 promoter polymorphisms but not dupA-H. pylori correlate to duodenal ulcers in H. pylori-infected females

Table 1 The PCR primers used in the study

SNP/gene	Primer sequence (5' →3')	Size (bp)	Restriction enzyme	Reference
MMP-3 _{-1612 5A/6A}	GATTACAGACATGGGTCACG	120	Xmn I	Shibata et al, 2005
	TTTCAATCAGGACAAGACGAAGTTT		6A: 120 bp
			5A: 97 bp + 23 bp
MMP-7 _{-181 A/G}	TGGTACCATAATGTCCTGAAT	150	EcoR I	Jormsjö et al, 2001
	TCGTTATTGGCAGGAAGCACACAATGAATT		A: 150 bp
			G: 120 bp + 30 bp
MMP-9 _{exon6 A/G}	CCATCCATGGGTCAAAGAAC	295	Sma I	Shibata et al, 2005 *
	GGGCTGAACCTGGTAGACAG		A: 295 bp
			G: 192 bp + 103 bp
TIMP-1 _{372 C/T}	GCACATCACTACCTGCAGTC	175	BssSI	Wollmer et al, 2002
	GAAACAAGCCCACGATTTAG		T: 175 bp
			C: 152 bp + 23 bp
TIMP-2 _{-418 G/C}	CGTCTCTTGTTGGCTGGTCA	304	BsoBI	Zhou et al, 2004
	CCTTCAGCTCGACTCTGGAG		C: 253 bp + 51 bp
			G: 230 bp + 51 bp + 23 bp
jhp0917 _1	TGGTTTCTACTGACAGAGCGC	307	-	Lu et al., 2005
jhp0917 _2	AACACGCTGACAGGACAATCTCCC
jhp0917 _3	GCCTAAGACCTCAAACTAGC	296	-	This study *
jhp0917 _4	CATTCTGTCAAGAGCTACC
jhp0918 _1	CCTATATCGCTAACGCGCGCTC	276	-	Lu et al.,
jhp0918 _2	AAGCTGAAGCGTTTGTAACG			2005
jhp0918 _3	GCTAGAAAGATCAACGGAAC	216	-	This study *
jhp0918 _4	CACTTGTCTGGCTCTCAT

*The primers were self-designed by applying Primer 3 and the restriction enzyme was selected based on the reference of Shibata et al, 2005.

ISSN: 1471-2180