Identification, expression and serological evaluation of the recombinant ATP synthase beta subunit of Mycoplasma pneumoniae

Table 1 Antigenic proteins^a identified in this study

Spot no.^b	Gene no.^c	Protein name	No. of matching peptides	Sequence coverage (%)	pI^d	Mass (Da)^d
1	MPN141	Adhesin P1	24	16	6.4	176.2
2	MPN573	Heat shock protein GroEl	30	59	5.5	58.1
3	MPN606	Enolase	14	45	6.1	49.3
4	MPN598	ATP synthase beta subunit	29	80	5.4	52.3
5	MPN392	Pyruvate dehydrogenase E1 β subunit	21	57	6.2	40.6
6	MPN025	Fructose bisphosphate aldolase	10	44	6.4	31.3

^a Antigenic proteins were separated by 2-DE, and their identities were determined by peptide mass fingerprinting.
^b Spot numbers are shown in Fig. 1.
^c Gene number in M. pneumoniae M129.
^d Values for pI and mass are theoretical values from the deduced amino acid sequence of the identified gene and open reading frame, respectively.

ISSN: 1471-2180