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Figure 2 | BMC Microbiology

Figure 2

From: RNA Interference inhibits Hepatitis B Virus of different genotypes in Vitro and in Vivo

Figure 2

The expression profile of four major interferon stimulated genes (ISGs) in shRNA plasmids transfected cells. Cytoplasmic RNAs, from Huh7 cells treated with or without IFNα-2a or transfected with either pSUPER vector or shRNA plasmids, were analysed by realtime RT-PCR for IFN stimulated genes STAT1, OAS1, GBP1 and MX1. The values on the figure, plotted as "Relative gene expression level" on the y-axis, were calculated as the mRNA levels of ISGs divided by the GAPDH (control) mRNA level. Student t test was used to assess the difference between shRNA plasmids (including empty pSUPER vector) of transfected cells and non-transfected cells (mock). No significant difference was observed.

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