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Table 1 List of Cryptosporidium genes selected for this study.

From: Multi-locus analysis of human infective Cryptosporidium species and subtypes using ten novel genetic loci

Primer name Gene function (CryptoDB) Sequence Tm (°C) Annealing temperature (°C) Size of amplified fragment
cgd2_80 F ABC transporter family protein GGA TTG GGG GTG ATA TGT TG 68 60 266 bp
cgd2_80 R   ACC TCC AAG CTG TGT TCC AG 70   
cgd6_200 F Oocyst wall protein 8 CGT TCC AAC AAT GGT GTG TC 68 60 447 bp
cgd6_200 R   GCA GCT GGA GTG CAA TCA TA 68   
cgd8_2370 F Adenosine kinase like ribokinase CAG GAA TTG CTC ACG GAA AT 66 60 685 bp
cgd8_2370 R   CCT TAA ATG CAT CCC CAC AG 68   
Chro.50317 F RNA polymerase A/beta'/A'' subunit GAT TTT GAT GGA GGG TCT CG 68 60 752 bp
Chro.50317 R   CTG GCA GCT TCA ACA CCA TA 68   
Chro.30149 F Ubiquitin-protein ligase 1 GGG ATT AGA TGC AGG TGG TG 70 60 331 bp
Chro.30149 R   TGG ATG CTC CAG CAT TAC AT 66   
Chro.50457 F Erythrocyte membrane-associated antigen CCT TTG GAT TGT CCC GAA TA 66 60 394 bp
Chro.50457 R   CAA TGC CAT ATG ATT TGA GAA AAA 65   
cgd6_5020 F Protein with WD40 repeats AAC AGG AGC TGA CGA TTG CT 60.4 57 271 bp
cgd6_5020 R   ACA TTG TGC CAT TCC AAG GT 58.35   
cgd2_2430 F Ximpact ortholog conserved protein seen in bacteria and eukaryotes GTA ACG CAT GGC GAA CCT AT 60.4 57 389 bp
cgd2_2430 R   AAG ATC AGC CTT GCA GCA TT 58.35   
Chro.20156 F Hypothetical protein TTC GCT TGA AGC CGT AAA CT 58.35 57 247 bp
Chro.20156 R   GGC ATT GAT ACC AGG CAA GT 60.4   
Chro.50330 F Leucyl tRNA synthetase TCG GTA CAG CAT CAG GTT CA 60.4 57 368 bp
Chro.50330 R   GTT TTT GCT CCC CCA GTT TT 58.35   
Cry-15 Oocyst wall protein gene [16] GTA GAT AAT GGA AGA GAT TGT G 57.08 60 555 bp
Cry-9   GGA CTG AAA TAC AGG CAT TAT CTT G 61.3   
  1. Gene name and annotation is according to CryptoDB. For each gene, a set of primers was designed. Primer name is the gene name followed by F or R (for forward and reverse, respectively). For each gene, primer sequences, annealing temperature and PCR product size are detailed.