Table 3 Primer sequences and annealing temperatures for ail and foxA.
From: Possible use of ail and foxA polymorphisms for detecting pathogenic Yersinia enterocolitica
Target gene and primer direction | Primer Sequences (5'→ 3') | GenBank no. | Location (nt) | Amplicon length | Annealing temp. | |
|---|---|---|---|---|---|---|
ail | Forward | GGT TAT TGT ATT AGT ATT GTT | M29945 | 446-466 | 585 bp | 57°C |
| Â | Reverse | CAG GTG GGT TTT CAC TAT CTG | Â | 1031-1051 | Â | Â |
foxA | Forward | CTC TGC GGA AGA TAA CTA TG | X60447 | 389-408 | 1532 bp | 58°C |
| Â | Reverse | ATC CGG GAA TAA ACT TGG CGT A | Â | 1899-1920 | Â | Â |