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Figure 4 | BMC Microbiology

Figure 4

From: In Helicobacter pylori auto-inducer-2, but not LuxS/MccAB catalysed reverse transsulphuration, regulates motility through modulation of flagellar gene transcription

Figure 4

Mutation of luxS Hp causes altered flagellin and hook protein production. Cell lysates of the strains indicated were subjected to immunoblotting with anti-flagellin (FlaA and FlaB) and anti-hook protein (FlgE) together [32]. The proteins were measured in wild-type, ΔluxSHp, ΔluxSHp+ cultures grown in Brucella broth at 37°C for 24 h. H. pylori strain 17874 wild-type [29] served as the positive control. Mutants in flaA (a kind gift from Paul O'Toole) and flgE [30] derived from this strain (17874ΔflaA and 17874ΔflgE) served as negative controls for identifying FlaA and FlgE, respectively.

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BMC Microbiology

ISSN: 1471-2180

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