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Figure 2 | BMC Microbiology

Figure 2

From: In Helicobacter pylori auto-inducer-2, but not LuxS/MccAB catalysed reverse transsulphuration, regulates motility through modulation of flagellar gene transcription

Figure 2

AI-2, but not cysteine rescues the motility defect of the ΔLuxS Hp mutant. (A) Wild-type, ΔluxSHp, and ΔluxSHp+ bacteria were seeded onto soft plates composed of normal medium. The non-motile ΔflhB mutant served as the negative control. (B) Wild-type, ΔluxSHp and ΔflhBHp bacteria were seeded onto motility plates supplemented with in vitro synthesised AI-2. Wild-type and ΔluxSHp were also seeded on motility plates containing buffer control solution used for in vitro AI-2 synthesis. (C) Wild-type, ΔluxSHp ΔmccAHp and ΔmccBHp strains were seeded onto chemically defined motility plates supplemented with cysteine. After 5 days of incubation, the motility halo of each strain on each plate was recorded using a digital camera and the area of each halo was measured using a GS-800 Calibrated Densitometer (Biorad).

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BMC Microbiology

ISSN: 1471-2180

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