Table 3 Oligonucleotide primers used in this study
From: The chitobiose transporter, chbC, is required for chitin utilization in Borrelia burgdorferi
Primer Name | Sequence (5'→3') |
|---|---|
5'BB0002mutF (KpnI) | GCTAGGGTACCACATTGCCTTTATCGGAATATTGACATC |
5'BB0002mutR (XbaI) | GCTAGTCTAGAAAGATGCGGAGCAGACAAAGGGAT |
pKFSS1 R1 | TGATGAACAGGGTCACGTCGTC |
3' BB0002mutF (XmaI) | GCTAGCCCGGGCGATATTAAGCTCTTGAACATTCTTAAA |
3'BB0002mutR (SacII) | GCTAGCCGCGGTAGTGCTATTAGTGCTTTATCTTTATTG |
5'BB0620mutF3 (KpnI) | GCTAGGGTACCTACTTTGAATTTTGAATATGGAG |
5'BB0620mutR2 (SalI) | GCTAGGTCGACTACCCAAATCAATCAATCAC |
pBSV2 R1 | TTATTATCGTGCACTCCTCCCGGT |
3'BB0620mutF2 (SacII) | GCTAGCCGCGGCGTATCCCAAAAATCAATAGAAAA |
3'BB0620mutR2 (AatII) | GCTAGGACGTCATGCAATCACCGCAATAGAAGCGG |
5'BBB04mutF2 (BamHI) | GCTAGGGATCCGAATAAGTAGCTTTACGTCT |
5'BBB04mutR2 (PstI) | GCTAGCTGCAGTACCAACAGTGGTATGTTGA |
3'BBB04mutF1 (XmaI) | GCTAGCCCGGGCCAATTTTGCTAGCAATAGGA |
3'BBB04mutR1 (SacII) | GCTAGCCGCGGGCATCTGGATTTAGGTCTGCTTTGA |
BBB04 complement F1 | GCTTCATTACTTCAACAGGACGACG |
BBB04 complement R1 | TCGCTAAGGCGTGTCTCAGCAATA |
chbC F1 | GGGAATTCAGCCCAATTCATGGTTTCC |
chbC R1 | GGCGGAACAGACTCTGGAAGCTTAAT |
BB0002 CF1 | ATGGACTTTTTAAAAACCTTTTCTTTTTTGTTTTTTAGC |
BB0002 CR1 | CTAAGGAATGAGTACTATATTGACACCCGA |
BB0620 mut confirm F1 | TCAAGAGTGGTATTGCCGTGTCCT |
BB0620 mut confirm R1 | ACTTGAACCCACGACAACTCGGAT |
BBB04 mut confirm F1 | AGCAGCATCTCCACCGTAAGGTAT |
BBB04 mut confirm R1 | CACCAGAGTAAGCTACAACAGGCA |