From: The chitobiose transporter, chbC, is required for chitin utilization in Borrelia burgdorferi
Strain or Plasmid | Genotype and Description | Reference |
---|---|---|
Strains | Â | Â |
   B. burgdorferi |  |  |
B31-A | High passage non-infectious wild type | [42] |
RR04 | StrR; B31-A putative β-N-acetylhexosaminidase (bb0002) mutant | This study |
RR53 | KanR; B31-A putative β-glucosidase (bb0620) mutant | This study |
RR60 | StrR KanR; B31-A double mutant for bb0002 and bb0620 | This study |
RR34 | StrR; B31-A chbC mutant | This study |
JR14 | StrR KanR; RR34 complemented with BBB04/pCE320 | This study |
A74 | CoumR; B31-A rpoS mutant | [42] |
   E. coli |  |  |
DH5α | supE 44 F- Δlac U169 (ϕ80lac Z ΔM15) hsdR 17 relA 1 endA 1gyrA 96 thi-1relA 1 | [43] |
Plasmids | Â | Â |
   pKFSS1 | StrR; B. burgdorferi shuttle vector, cp9 based | [37] |
   pBSV2 | KanR; B. burgdorferi shuttle vector, cp9 based | [38] |
   pCE320 | KanR ZeoR; B. burgdorferi shuttle vector, cp32 based | [40] |
   pBB0002.7 | StrR; aadA::bb0002 | This study |
   pBB0620.5 | KanR; kan::bb0620 | This study |
   pBBB04.5 | StrR; aadA::bbb04 | This study |
   BBB04/pCE320 | KanR; bbb04 complementation construct | This study |