Skip to main content

Table 2 Strains and plasmids used in this study.

From: The chitobiose transporter, chbC, is required for chitin utilization in Borrelia burgdorferi

Strain or Plasmid Genotype and Description Reference
Strains   
   B. burgdorferi   
B31-A High passage non-infectious wild type [42]
RR04 StrR; B31-A putative β-N-acetylhexosaminidase (bb0002) mutant This study
RR53 KanR; B31-A putative β-glucosidase (bb0620) mutant This study
RR60 StrR KanR; B31-A double mutant for bb0002 and bb0620 This study
RR34 StrR; B31-A chbC mutant This study
JR14 StrR KanR; RR34 complemented with BBB04/pCE320 This study
A74 CoumR; B31-A rpoS mutant [42]
   E. coli   
DH5α supE 44 F- Δlac U169 (ϕ80lac Z ΔM15) hsdR 17 relA 1 endA 1gyrA 96 thi-1relA 1 [43]
Plasmids   
   pKFSS1 StrR; B. burgdorferi shuttle vector, cp9 based [37]
   pBSV2 KanR; B. burgdorferi shuttle vector, cp9 based [38]
   pCE320 KanR ZeoR; B. burgdorferi shuttle vector, cp32 based [40]
   pBB0002.7 StrR; aadA::bb0002 This study
   pBB0620.5 KanR; kan::bb0620 This study
   pBBB04.5 StrR; aadA::bbb04 This study
   BBB04/pCE320 KanR; bbb04 complementation construct This study