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Table 2 Strains and plasmids used in this study.

From: The chitobiose transporter, chbC, is required for chitin utilization in Borrelia burgdorferi

Strain or Plasmid

Genotype and Description

Reference

Strains

  

   B. burgdorferi

  

B31-A

High passage non-infectious wild type

[42]

RR04

StrR; B31-A putative β-N-acetylhexosaminidase (bb0002) mutant

This study

RR53

KanR; B31-A putative β-glucosidase (bb0620) mutant

This study

RR60

StrR KanR; B31-A double mutant for bb0002 and bb0620

This study

RR34

StrR; B31-A chbC mutant

This study

JR14

StrR KanR; RR34 complemented with BBB04/pCE320

This study

A74

CoumR; B31-A rpoS mutant

[42]

   E. coli

  

DH5α

supE 44 F- Δlac U169 (ϕ80lac Z ΔM15) hsdR 17 relA 1 endA 1gyrA 96 thi-1relA 1

[43]

Plasmids

  

   pKFSS1

StrR; B. burgdorferi shuttle vector, cp9 based

[37]

   pBSV2

KanR; B. burgdorferi shuttle vector, cp9 based

[38]

   pCE320

KanR ZeoR; B. burgdorferi shuttle vector, cp32 based

[40]

   pBB0002.7

StrR; aadA::bb0002

This study

   pBB0620.5

KanR; kan::bb0620

This study

   pBBB04.5

StrR; aadA::bbb04

This study

   BBB04/pCE320

KanR; bbb04 complementation construct

This study