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Table 2 Efficiencies of pRKaraRed-mediated scarless modification to different targets

From: Scarless and sequential gene modification in Pseudomonas using PCR product flanked by short homology regions

Target Size (bp) Positive colonies/Growing colonies (%)a Overall efficiency (%)
   Replacement using sacB-bla cassetteb Deletion of sacB-bla cassettec  
A. Deletion of genes
rsm A 186 43/44 (98%) 19/20 (95%) 93%
las I 606 53/54 (98%) 20/20 (100%) 98%
gac A 645 49/50 (98%) 18/20 (90%) 88%
qsc R 714 36/37 (97%) 19/20 (95%) 92%
las R 720 56/57(98%) 20/20 (100%) 98%
rhl R 762 59/61(97%) 20/20 (100%) 97%
phz M 1005 65/68 (96%) 19/20 (95%) 91%
rpo S 1005 46/47 (98%) 20/20 (100%) 98%
phz S 1209 70/72 (97%) 20/20 (100%) 97%
phz H 1833 68/69 (99%) 19/20 (95%) 89%
rpo D 1854 52/54 (96%) 20/20 (100%) 96%
pts P 2280 78/80 (98%) 19/20 (95%) 93%
B. Single-point mutation
phz S 1 24/26 (94%) 19/20 (95%) 89%
(A761T)     
C. Deletion of operons
phz A1-G1 6267 47/50 (94%) 19/20 (95%) 89%
phz A2-G2 6273 61/63 (97%) 20/20 (100%) 97%
  1. a. Determined by PCR amplification and DNA sequencing
  2. b. Screening of CarbRSucS colonies
  3. c. Screening of CarbSSucR colonies