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Table 2 Sampling methods compared in this study.

From: Sampling and pyrosequencing methods for characterizing bacterial communities in the human gut using 16S sequence tags

    days at -80C
Method Identifier Storage Method DNA Purification Method min max
1 Immediately frozen (-80°C) Qiagen Stool 2 14
2 Immediately frozen (-80°C, sampled 1 cm from sample 1) Qiagen Stool 6 63
3 Immediately frozen (-80°C) MoBio PowerSoil 58 72
4 4C for 24 h, then frozen (-80°C) Qiagen Stool 1 21
5 4C for 48 h, then frozen (-80°C) Qiagen Stool 0 12
6 PSP for 48 h, then frozen (-80°C) PSP 0 12
7 Immediately frozen (-80°C) Qiagen Stool (70°C) 7 7
8 Fresh Qiagen Stool 0 0
9 Immediately frozen (-80°C) Hot phenol with bead beating 118 137