Table 2 Sampling methods compared in this study.
| Â | Â | Â | days at -80C | |
|---|---|---|---|---|
Method Identifier | Storage Method | DNA Purification Method | min | max |
1 | Immediately frozen (-80°C) | Qiagen Stool | 2 | 14 |
2 | Immediately frozen (-80°C, sampled 1 cm from sample 1) | Qiagen Stool | 6 | 63 |
3 | Immediately frozen (-80°C) | MoBio PowerSoil | 58 | 72 |
4 | 4C for 24 h, then frozen (-80°C) | Qiagen Stool | 1 | 21 |
5 | 4C for 48 h, then frozen (-80°C) | Qiagen Stool | 0 | 12 |
6 | PSP for 48 h, then frozen (-80°C) | PSP | 0 | 12 |
7 | Immediately frozen (-80°C) | Qiagen Stool (70°C) | 7 | 7 |
8 | Fresh | Qiagen Stool | 0 | 0 |
9 | Immediately frozen (-80°C) | Hot phenol with bead beating | 118 | 137 |