Figure 1

Morphology of Chlamydia pecorum mono- and co-infection with PEDV. a) Vero cells were infected with Chlamydia pecorum 1 MOI for 39 h, with subsequent PEDV inoculation and labelled with an anti-Chlamydia antibody (green); b) double infected monolayer were labelled for ca-PEDV in red, Chlamydia in green and DNA in blue; c) Chlamydia pecorum mono-infected Vero cells labelled with an anti-Chlamydia antibody (green) and DNA staining (blue); d) Inclusion size was measured as described and the frequency of chlamydial inclusions assembled into sizes of 50 μm² area groups depicted. The difference between mono and double infected monolayers was statistically analyzed using students t-test. The groups were significantly different with p = 0.0044.