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Figure 1 | BMC Microbiology

Figure 1

From: Exocytosis and protein secretion in Trypanosoma

Figure 1

General purification procedure. Trypanosomes were intraperitoneally injected into rats. When their multiplication reached the logarithmic growth stage, parasites were purified from blood by chromatography and resuspended in secretion medium. After 2 h, parasites were removed by centrifugation and secreted proteins (ESPs) were purified through chromatography. ESPs were separated on polyacrylamide gel electrophoresis (PAGE), stained before mass spectrometry (MS/MS) analysis.

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BMC Microbiology

ISSN: 1471-2180

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