Skip to main content

Advertisement

Table 1 Primers used for preparation of probes

From: Chromosomal instability in Streptomyces avermitilis: major deletion in the central region and stable circularized chromosome

Probes Primers sequence (5'-3') Description
W 11 GTTGCGGACGTGTGACTTG to detect the left extremity of chromosome
  12 GAACTACATGCCGGGAGTG  
aveC 15 CAGCAAGGATACGGGGAC to detect the avermectin biosynthetic gene cluster
  16 ACCGAGCACGATGCCGATG  
G1-139 139 GGTTGACGACGTCCTTGAG to detect fragment Ase I-G1
  140 CGACACTCATGAAGCGACC  
G2-152 152 CTGCTCAAGACGAAGGTGC to detect fragment Ase I-G2
  153 CCGTCACATCGCTGTCATG  
D600 131 GTGATCGTGAAGACCTCGC to detect fragment Ase I-D
  132 CTCCACCATGACAAGACCG  
Dr 19 AGTCGTACGTCCGCAACTG to detect the right extremity of chromosome
  20 AGGTCTTCCGCTTCGCTTC  
D20 125 CTCTAGACGGCGGAATCAC to detect the duplication and translocation of right 88-kb end
  126 GCGACAAGGGCTAAGACTC  
D60 96 GTTCTGGCAGTCGTCGTAG to detect the duplication and translocation of right 88-kb end
  97 TGAAGAAGACCCGGTCTGG  
D80 98 ACGAACGTGCCCTGCTCAC to detect the duplication and translocation of right 88-kb end
  99 GGTGACGAGTTCGGAGACG  
N1 75 GGAGGTAGCGGATGTTGTG to detect the rearrangement event in fragment Ase I-NA1
  76 CTGGTCCCAGTACACGATC  
N2 236 GGCTCGTTCATCTTCCTCG to detect the rearrangement event in fragment Ase I-NA2
  239 GCACATCAGAGGGTCATGC  
N3 272 CGTTGACGTAGAGCTGCG to detect the rearrangement event in fragment Ase I-NA3
  248 ACCTGAGCAGCTCGTGAAG  
N4 405 TGTGACGGTGTGCCAGTAG to detect the rearrangement event in fragment Ase I-NA4
  406 ATGCCCTCGACTACGACAC