Response of the B. cereus lysK T-box regulatory element to reduced levels of charged tRNAAsn. A) The mixed codon box for lysine and asparagine. (B) Growth (open symbols) and β-galactosidase activity (closed symbols) of NF60 (Pspac asnS P
lacZ pMAP65) in LB containing 1 mM (diamonds) and 600 μM (triangles) IPTG. (C) Northern analysis of tRNALys charging in wild-type B. subtilis strain 168 and strain NF60 growing in LB media with the indicated IPTG concentrations. The percentage of charged tRNALys is indicated beneath each lane. The profiles presented are representative.