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Figure 1 | BMC Microbiology

Figure 1

From: The T box regulatory element controlling expression of the class I lysyl-tRNA synthetase of Bacillus cereus strain 14579 is functional and can be partially induced by reduced charging of asparaginyl-tRNAAsn

Figure 1

Response of the B. cereus lysK T-box regulatory element to reduced tRNALys charging. Growth is represented by open symbols and β-galactosidase activity by closed symbols. Representative expression profiles are presented. Growth is represented by open symbols and β-galactosidase activity by closed symbols. (A) Growth and β-galactosidase accumulation in strain NF33 (PlysK(T box)lacZ) in minimal media. Strain NF33 was grown in minimal medium containing 100 μg/ml lysine (triangles) and 20 μg/ml lysine (squares). (B) Growth and β-galactosidase accumulation in strain BCJ367 (P lysK Tbox lacZ Pspac lysS pMap65) in LB containing varying IPTG concentrations: 1 mM IPTG (diamonds); 250 μM IPTG (squares) and 100 μM IPTG (triangles). (C) Growth and β-galactosidase activity of strain BCJ367 in LB containing 100 μM IPTG. The IPTG concentration was increased to 1 mM at 150 minutes, indicated by the arrow.

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