Southern blot hybridization of Eco RI-restricted M. suis DNA showing the genomic location of the ms 262 clone insert on a 1.2- and a 2.7-kb fragment. (A) agarose gel electrophoresis of Eco RI-restricted DNA. (B) the blot probed with the DIG-labeled 950 bp-Eco RI fragment of the library clone ms 262; (C) the blot probed with the DIG-labeled 1050 bp-Eco RI fragment of the library clone ms 262; (M) molecular weight standard; (Ms) M. suis. The arrows indicate the positions of the hybridized 1.2- and 2.7-kb fragments. (D) schematic map of the ORF localisation on the library clone ms 262. The grey box arrows indicate the two ORFs: ppa (inorganic pyrophosphatase) and trx (thioredoxin).