Common origin of plasmid encoded alpha-hemolysin genes in Escherichia coli

BMC Microbiology

Table 3 Detection of α-hly plasmid specific sequences in porcine STEC strains.

			Size of PCR products with primers^a
Serotype	No. strains	Plasmid group	1f+1r	32f + 32r	44 f + 44r	99 f + 99r
O138:H14^b	4	3	678	2900	1500	650
O139:H1^b	9	3	678	2900	1500	650
O139:H1^b	1	single	678	2000	1500	650
O141:H4^b	7	3	678	2900	1500	650
O141:H4^b	3	2	678	2000	685	650
O2:H32^c	1	2	678	2000	685	650
O36:H19^c	1	2	678	2000	685	650

a) size of the PCR products (bp) with primers 1f/r, 32f/r, 44f/r and 99f/r as described in Tables 1+ 2. Digestion of PCR products by HinfI resulted in identical patterns between strains corresponding to the size of the PCR products obtained (Table 1)
b) Strains isolated from feces of diseased pigs [29]
c) Strains isolated from pork meat [29]

ISSN: 1471-2180