Common origin of plasmid encoded alpha-hemolysin genes in Escherichia coli

BMC Microbiology

Table 1 Relevant properties of strains carrying plasmid and chromosomally encoded α-hly determinants

					PCR products with primers pairs^a
strain	Serotype^b	Origin, reference^d	hly -plasmid (kb)	Plasmid group	1f/r (678 bp)	32f/r (671 bp)	44f/r (685 bp)	99f/r (650 bp)	72f/r (695 bp)	81f/r (773 bp)
C4115	O26:[H11]	human, EPEC [21]	pEO5 (157)	1	+	+	+	+	-	-
TPE422	Or:H48	E. coli K12 (pEO5) [21]	pEO5 (157)	1	+	+	+	+	-	-
CB9866	O26:[H11]	cattle, EPEC [21]	pEO5 (157)	1	+	+	+	+	-	-
CB1027	O26:[H11]	human, EPEC [21]	pEO5 (157)	1	+	+	+	+	-	-
CB1030	O26:[H11]	human, EPEC [21]	pEO5 (157)	1	+	+	+	+	-	-
IP187	O26:[H11]	human, EPEC [21]	pEO5 (157)	1	+	+	+	+	-	-
84/2195	Ont:H10	dog [10]	pEO9 (146)	1	+	+	+	+	-	-
84-R	O121:H46	dog [10]	pEO13 (97)	1	+	+	+	+	-	-
374	Or:H48	mouse [24]	pHly152 (48)	2	+	e)	+	+	-	-
84-3208	O42:H37	dog, ETEC[10]	pEO11 (48)	2	+	e)	+	+	-	-
84-2573	O70:NM	dog, ETEC [10]	pEO12 (48)	2	+	e)	+	+	-	-
CB853	O138:H14	pig, STEC [29]	pEO853 (145)	3	+	f)	g)	+	-	-
CB855	O138:NM	pig, STEC [29]	pEO855 (140)	3	+	f)	g)	+	-	-
CB857	O157:NM	pig, ETEC [42]	pEO857 (97)	3	+	f)	g)	+	-	-
CB860	O149:H10	pig, ETEC [42]	pEO860 (48)	single	+	+	g)	+	-	-
84-2S	O75:H2	dog [10]	pEO14 (97)	single	-	-	-	-	-	-
536^h	O6:K15:H31	human UPEC [20]	-	n.a	-	-	-	-	+	+
536-14	O6:K15:H31	PAI I deletion mutant of 536 [20]	-	n.a	-	-	-	-	+	-
695/83	O126:H27	human [19]	-	n.a	-	-	-	-	-	i)
J96^h	O4:K6	human UPEC [46]	-	n.a	-	-	-	-	+	j)
KK6-16	E. cloacae	human [26]	-	n.a	k)	-	-	-	-	-

a) primer pairs and size of the PCR products obtained with strains TPE422 (pEO5) (primers 1f/r, 32f/r and 44f/r) and 536 (primers 81f/r and 72f/r) (see Table 2).
+ = a PCR product of the same size as obtained with strains TPE422 (pEO5) or 536, respectively.
- = no PCR product obtained
PCR products with other sizes than obtained with the reference strains are indicated for their length in bp.
b) O:H serotype, Or = rough LPS, Ont = O-antigen not typable, NM = non motile
c) α = alpha-hemolytic (α-hly genes), e-hly = enterohemolytic (EHEC-hly genes)
d) All strains were from feces if not otherwise indicated, ETEC = enterotoxigenic E. coli, STEC = shigatoxigenic E. coli, UPEC = uropathogenic E. Coli
e) 2000 bp PCR product (2007 bp as calculated from the nucleotide sequence of pEO11 [GenBank FM210249]
f) 2900 bp PCR product (2868 bp as calculated from the nucleotide sequence of pEO860) [GenBank FM210351]
g) 1500 bp PCR products
h) strain carries two α-hly determinants in the chromosome
i) 950 bp PCR product
j) 860 bp PCR product
k) 778 bp PCR product (calculated from nucleotide sequencing of KK6-16 [GenBank FM210352])

ISSN: 1471-2180