Figure 8

Synthesis of trehalose by R. tropici CIAT 899 from different carbon sources. ¹H-NMR analysis of cellular extracts from R. tropici CIAT899 grown in 100 mM NaCl MAS medium containing glucose (A), galactose (B), mannose (C) or manitol (D) as a carbon source. T and Gl indicate the signals corresponding to the anomeric protons of the glucose units of trehalose and the cyclic glucan, respectively. (E) ¹³C-NMR spectra of intracellular solutes accumulated by R. tropici CIAT899 grown in 0.1 M NaCl MAS medium with ¹³C1/6 manitol as a carbon source. Abbreviations: T, trehalose; Gl, cyclic β-glucan; M, manitol; G, glutamate.