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Table 4 Number (percentages) of sequences and amplified in each of the most common Basidiomycete groups, from the original subset3 and from the amplification of ITS3-ITS4-B from subset3, allowing no or 3 mismatches.

From: ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases

  subset3 ITS3-4B_3mis ITS3-4B_0mis
Agaricales 361 269 (74.5) 118 (32.7)
Boletales 18 17 (94.4) 15 (83.3)
Cantharellales 33 31 (93.9) 0
Hymenochaetales 10 7 (70) 0
Polyporales 28 8 (28.6) 0
Russulales 97 64 (66.0) 0
Thelephorales 6 4 (66.7) 0
Dacrymycetes 1 0 0
Tremellomycetes 38 13 (34.2) 0
Pucciniomycotina 8 0 0
Ustilaginomycotina 21 0 0
Other categories * 71 21 (29.6) 3 (4.2)
  1. * 'Other categories' represent smaller orders including Agaricomycetidae.