Table 4 Number (percentages) of sequences and amplified in each of the most common Basidiomycete groups, from the original subset3 and from the amplification of ITS3-ITS4-B from subset3, allowing no or 3 mismatches.
From: ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases
subset3 | ITS3-4B_3mis | ITS3-4B_0mis | |
|---|---|---|---|
Agaricales | 361 | 269 (74.5) | 118 (32.7) |
Boletales | 18 | 17 (94.4) | 15 (83.3) |
Cantharellales | 33 | 31 (93.9) | 0 |
Hymenochaetales | 10 | 7 (70) | 0 |
Polyporales | 28 | 8 (28.6) | 0 |
Russulales | 97 | 64 (66.0) | 0 |
Thelephorales | 6 | 4 (66.7) | 0 |
Dacrymycetes | 1 | 0 | 0 |
Tremellomycetes | 38 | 13 (34.2) | 0 |
Pucciniomycotina | 8 | 0 | 0 |
Ustilaginomycotina | 21 | 0 | 0 |
Other categories * | 71 | 21 (29.6) | 3 (4.2) |