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Table 2 Percentage of sequences amplified in silico, allowing one mismatch, from ascomycetes, basidiomycetes and 'non-Dikarya' with different primer combinations and using the three sequence subsets 1-3 (see Material and Methods) as templates.

From: ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases

Data subsets Primer comb. Ascomycetes Basidiomycetes 'non-Dikarya'
Subset 1 ITS1*-ITS2 61.21 86.21 88.57
  ITS1-F*-ITS2 90.75 99.14 92.38
  ITS5*-ITS2 90.84 99.14 98.10
Subset 2 ITS1*-ITS4 61.91 82.00 84.86
  ITS3*-ITS4 98.39 73.91 91.04
  ITS5-ITS2* 94.89 72.10 92.63
Subset 3 ITS3-ITS4* 94.71 85.55 98.49
  ITS3-ITS4-B* - 39.31 -
  1. * primer evaluated for mismatches within each pair