Table 2 Percentage of sequences amplified in silico, allowing one mismatch, from ascomycetes, basidiomycetes and 'non-Dikarya' with different primer combinations and using the three sequence subsets 1-3 (see Material and Methods) as templates.
From: ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases
| Data subsets | Primer comb. | Ascomycetes | Basidiomycetes | 'non-Dikarya' |
|---|---|---|---|---|
| Subset 1 | ITS1*-ITS2 | 61.21 | 86.21 | 88.57 |
| ITS1-F*-ITS2 | 90.75 | 99.14 | 92.38 | |
| ITS5*-ITS2 | 90.84 | 99.14 | 98.10 | |
| Subset 2 | ITS1*-ITS4 | 61.91 | 82.00 | 84.86 |
| ITS3*-ITS4 | 98.39 | 73.91 | 91.04 | |
| ITS5-ITS2* | 94.89 | 72.10 | 92.63 | |
| Subset 3 | ITS3-ITS4* | 94.71 | 85.55 | 98.49 |
| ITS3-ITS4-B* | - | 39.31 | - |