ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases

BMC Microbiology

Table 1 Number of plant and fungi ITS sequences amplified in silico from EMBL fungal and plant databases, using the various primer combinations and allowing none to three mismatches.

* The number of mismatches allowed between the primer and the DNA strand reflects the stringency level of the PCR, i.e. strict PCR conditions such as annealing temperature close to or above the recommended Tm will not allow unspecific sequences (including one or more mismatches) to be amplified.

ISSN: 1471-2180