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Table 1 Number of plant and fungi ITS sequences amplified in silico from EMBL fungal and plant databases, using the various primer combinations and allowing none to three mismatches.

From: ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases

Primer comb. Fungal ITS sequences Plant ITS sequences
Number of mismatches * 0 1 2 3 0 1 2 3
ITS5-ITS4 5482 5924 6026 6123 500 514 5667 5996
NS7-ITS2 1067 1291 1313 1320 23 190 231 403
ITS3-LR3 2070 2459 2499 2548 51 168 248 300
ITS1-ITS2 17545 19816 25223 25457 1107 17665 18755 19084
ITS1-F-ITS2 2112 4169 4592 4658 20 21 21 24
ITS5-ITS2 7713 8993 9180 9293 94 703 11123 12100
ITS1-ITS4 10013 10610 12488 12656 5783 6740 7500 7620
ITS3-ITS4 18815 21195 21663 22078 415 7829 8583 8852
ITS3-ITS4-B 1269 1673 1811 1863 0 0 0 0
  1. * The number of mismatches allowed between the primer and the DNA strand reflects the stringency level of the PCR, i.e. strict PCR conditions such as annealing temperature close to or above the recommended Tm will not allow unspecific sequences (including one or more mismatches) to be amplified.