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Figure 7 | BMC Microbiology

Figure 7

From: Robustness analysis of culturing perturbations on Escherichia coli colony biofilm beta-lactam and aminoglycoside antibiotic tolerance

Figure 7

Effect of AI-2 quorum sensing circuit gene deletions on antibiotic tolerance of E. coli biofilm cultures. Cells were grown as biofilms for 6 hours before being transferred to treatment plates for 24 hours. Reported cfu/biofilm data was determined after treatment. 7a) Cultures grown at 37°C on LB only medium. 7b) Cultures grown at 37°C on LB and 10 g/L glucose. ΔluxS mutant lacked gene for AI-2 synthesis, ΔlsrK mutant lacked gene for AI-2 phosphorylation, ΔlsrR mutant lacked gene for lsr operon repression, and ΔlsrF mutant lacked gene for AI-2 degradation. Black bars = control, dark gray bars = kanamycin (100 ug/ml) challenge, light gray bars = ampicillin (100 ug/ml) challenge. Number at the base of each bar denotes the number of independent replicates. cfu = colony forming unit.

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