Table 2 Bacterial strains and plasmids used
Strains or plasmids | Relevant properties | Reference or Source |
|---|---|---|
Bacterial Strains | ||
E. coli DH5α | Δ lacU 169 hsdR 17 recA1 endA 1 gyrA 96 thiL relA1 | Gibco/BRL |
E. coli Bl21 λ (DE3) pLysS | omp T hsd S(r B- mB-) dcm+ Tetr endA gal λ (DE3) | Novagen |
A. brasilense Sp7 | Wild-type strain | [12] |
Plasmids | ||
pET15b | Expression vector, Ampr | Novagen |
pRKK200 | Kmr, Spr, lacZ-fusion reporter vector | [25] |
pSK7 | gca1 ORF from A. brasilense Sp7 cloned in Nde I/BamH I site of pET15b | This work |
pSJ3 | Amplicon A and B cloned in pSUP202 plasmid | This work |
pSJ4 | Kmr gene cassette cloned in BglII site of pSJ1. | This work |
pSK8 | A. brasilense argC promoter region cloned in Kpn I/Stu I site of pRKK200 | This work |
pSK9 | A. brasilense gca1 promoter region cloned in Kpn I/Stu I site of pRKK200 | This work |