Primer | Description | Sequence (5'-3') |
---|---|---|
Type 3 fimbriae deletion primers | Â | |
1293 | 50 bp overhang mrk knockout F-primer 1 | TCTTCTCTCTGCAGCAATGGCAACCGCGTTTTTTGGCATGACTGCTGCCCGTGTAGGCTGGAGCTGCTTCG |
1294 | 50 bp overhang mrk knockout R-primer 1 | GGTGTGAGCGGGATAGTTGTCTGAGTCACAGGCAGTTTCCTCTTCACCAGCATATGAATATCCTCCTTAG |
1295 | Knockout screening F 1 | GGCAGCATAACCGAACAAAT |
1296 | Knockout screening R 1 | TAAATTTTCTGCGGCAAACC |
1456 | 50 bp overhang mrk knockout F-primer 2 | GTTAACGGTACCCGCTTTATTTATCCAGGAAATGAAAAAGAAATAACGGTGTGTAGGCTGGAGCTGCTTCG |
1457 | 50 bp overhang mrk knockout R-primer 2 | CCTTTGTCCCAGAACTCCGGGCTGACATAGTTTTTCAGGCGTTGATCTTCCATATGAATATCCTCCTTAG |
1463 | Knockout screening R 2 | GGTCTGGTTGCTGTTCCAGT |
1458 | 50 bp overhang mrk knockout F-primer 3 | GAGAGCTGCACCGTTATTTCTTTTTCATTTCCTGGATAAATAAAGCGGGTGTGTAGGCTGGAGCTGCTTCG |
1459 | 50 bp overhang mrk knockout R-primer 3 | CTTTACCGGCGGTGATTTTCAGGTCGTAGCGCCCTTTCCATTCGCCGTTGCATATGAATATCCTCCTTAG |
1464 | Knockout screening F 3 | CTGCCGAGCTTAATACGCA |
1465 | Knockout screening R 3 | GCAACGCCTTTATCCCAGA |
1460 | 50 bp overhang mrk knockout F-primer 4 | CTGCCGTTCAGGACGGCGCGCGCTTCACTTACTACGTTGGCTACGCTACCGTGTAGGCTGGAGCTGCTTCG |
1461 | 50 bp overhang mrk knockout R-primer 4 | GTGTAGCAGACGCCCATTTTGCCGTCTTTTCCACGGGTGATATTCAGGTCCATATGAATATCCTCCTTAG |
1466 | Knockout screening F 4 | ATCCAGCTGGTTCTGTCCAC |
1467 | Knockout screening R 4 | CCGGGCTGACAAAATTCTTA |
1462 | 50 bp overhang mrk knockout F-primer 5 | GGCTGGATAACGGTAATGCTGACGCAACGCCGGATACTATTACGACTCCAGTGTAGGCTGGAGCTGCTTCG |
1468 | Knockout screening F 5 | AAAAGAAATAACGGTGCAGCTC |
Inverse PCR primers | Â | |
1450 | mrkA R-primer A | CTTTACCGAAGAAATTAACC |
1451 | mrkA R-primer C | GAAGAAATTAACCTGACCGCC |
1452 | mrkD F-primer A | AAACCTATCTGAGCGCCAAC |
1453 | mrkD F-primer B | CCTCTTATGACTGGGAGAACG |
1454 | mrkD F-primer C | TGCGCGCTTCTATCAATATG |
1455 | mrkD F-primer D | GGCGTCCAGGTACTGAAAGA |
Outside mrk cluster screening primers | Â | |
1469 | F-primer (outside mrkA of K. pneumoniae MGH78578) | TGGCGGTACATACTTCACTCA |
1470 | R-primer (outside mrkF of K. pneumoniae MGH78578) | ATCCTCTGCCTATTGTCTGACT |
1471 | F-primer (mrkE of K. pneumoniae pIA565) | TTTGCATATCCGCAATCTGA |
1472 | F-primer (outside mrkA of C. koseri ATCC BAA-895) | AATTGCAGGAACAGGGTCTG |
1473 | R-primer (outside mrkF of C. koseri ATCC BAA-895) | TTCCTTTCCCGTAACCA |
1474 | F-primer (outside mrkA of E. coli MS2027) | AGCGTTCTGCATGGCTTTAT |
1475 | R-primer (outside mrkF of E. coli MS2027) | AACGACGTTGGGTCGTTAGT |
pMAS2027 screening primers | Â | |
1476 | pMAS2027 screening primer F1 | GCGCAGAACTGGTTATGAAT |
1477 | pMAS2027 screening primer R1 | TCATGGATTTTCTTCCTTAACAA |
1478 | pMAS2027 screening primer F2 | ACAACTATCCCGCTCACACC |
1479 | pMAS2027 screening primer R2 | ACCGTTAACGCGTAGTCACC |
1480 | pMAS2027 screening primer F3 | TGCTTCAGCAGCATATCAGG |
1481 | pMAS2027 screening primer R3 | GGAAAGCGTTAAAGCAGGTG |
1482 | pMAS2027 screening primer F4 | CCGTATGCGCTTTTTCAAGT |
1483 | pMAS2027 screening primer R4 | AAAGTTGAAGCCCGCTTTCT |
1484 | pMAS2027 screening primer F5 | ACGGGTAAGACCGCTAACCT |
1485 | pMAS2027 screening primer R5 | TCGATAAGGTAGGCATCAACAA |