Interaction between the flagellar components using GST pull-down assays. A: GST- FlhA308-583 was bound to glutathione beads and was used to pull down either His-FliF35-341 or His-FliF1-271 from an E. coli lysate. Beads were harvested by centrifugation and washed with either 0 mM, 200 mM or 500 mM NaCl and probed for His-tagged protein by Western blot using anti-his antibody. GST- FlhA308-583 co-purified with His-FliF35-341 but not His-FliF1-271 while GST alone did not co-purify with either. GST-FlhA308-583 is shown as a loading control. B: Full length GST-FliI, GST-FliI1-400, or GST-FliI150-470 were bound to glutathione beads and were used to pull down His-FlhA308-583 from an E. coli lysate. Full length GST-FliI and GST-FliI1-400 were able to co-purify with FlhA308-583 while GST-FliI150-470 was not. GST alone was not able to co-purify with His-FlhA305-583. C: Full length GST-FliI was bound to glutathione beads and used to pull down His-FliF35-341 and His-FliF1-271. GST-FliI did not co-purify with either FliF fragment.