The role of iron uptake in pathogenicity and symbiosis in Photorhabdus luminescens TT01

BMC Microbiology

Table 4 Oligonucleotides used for construction of targeted deletion mutants.

Gene(s)	Sequence 5' to 3'*	Name
exbD	1. TTATGCATGC GGTGATTGCTTCTGTTATTACTT GG	RJW115
	2. GAATCAGTGACAATTACATAAGTCACCTTGTCTTG	RJW116
	3. CAAGGTGACTTATGTAATTGTCACTGATTCTTCC	RJW117
	4. TTATGAGCTC GCCAACCAATTTGCCTCTGCCCTAC	RJW118
yfeABCD	1. TTATGCATGC GGTTATCAATACCTGCCAGATGC	RJW171
	2. CCCTTTTTGTTACATAAATTCAAACC	RJW172
	3. GGTTTGAATTTATGTAACAAAAAGGGTTATATCTG	RJW173
	4. TTATGAGCTC GGTGTTGAAGTTTGTTACTTATAGC	RJW174
feoABC	1. TTATGCATGC CGTAGTAAAAGCGGGTGATATCG	RJW167
	2. GCTAATCATTTTCAATTCCTACATATGACCTTCCG	RJW168
	3. CGGAAGGTCATATGTAGGAATTGAAAATGATTAGC	RJW169
	4. TTATGAGCTC CCAAAACGCTTCTCTTAGAAGATGC	RJW170

*: the underlined sequence indicate the restriction endonuclease sites used for cloning the amplicon into pDS132.

ISSN: 1471-2180