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Table 3 CBC-LAMP assay sensitivity from cultured cells and infected tissue

From: Rapid and sensitive detection of Citrus Bacterial Canker by loop-mediated isothermal amplification combined with simple visual evaluation methods

Strain

Specimen

source

Detection method

CFU per reaction

(10-fold dilutions)

X. citri pv. citri

Pure culture

 

395.3

37.6

5.2

0.7

  

Gel

+

+

+

-

  

LFD

+

+

+

-

  

SYBRGreen

+

+

+

-

X. citri pv. citri

Infected tissue

 

248.4

18.7

3.3

0.2

  

Gel

+

+

-

-

  

LFD

+

+

-

-

  

SYBRGreen

+

+

-

-

  1. For each dilution the CBC-LAMP reaction was performed in triplicate. Gel: gel electrophoresis. LFD: lateral flow dipstick. +: Positive reaction.-: Negative reaction.