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Figure 4 | BMC Microbiology

Figure 4

From: LPS-induced IL-8 activation in human intestinal epithelial cells is accompanied by specific histone H3 acetylation and methylation changes

Figure 4

LPS induces early histone H3 methylation and acetylation changes at the promoter region of IL-8 gene. Chromatin from HT-29 cells was harvested at the indicated time points after LPS exposure. (A) Schematic representation of IL-8 promoter region, as in Figure 3. Positions of the primers used for ChIP analyses are shown. Presented are the results of ChIP analyses using anti-acetyl-H3 (B) anti-dimethyl-H3K4 (C), anti-dimethyl-H3K9 (D) and anti-trimethyl-K27 (E) antibodies. DNA sequences recovered after the indicated times of LPS treatment were quantified by real-time PCR using the primers indicated above. Average% input ± S.D from 4 independent experiments are plotted. *, p < 0.01; **, p < 0.05; n.s.= not significant, compared to control cells.

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